CA XII Antibody (D-2): sc-374314

CA XII Antibody (D-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects CA XII protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-CA XII antibody (D-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CA XII (carbonic anhydrase XII) is a transmembrane enzyme that plays a crucial role in regulating pH and bicarbonate levels in tissues, particularly in the kidney, colon, and pancreas. CA XII function is vital for maintaining acid-base balance and facilitating carbon dioxide and bicarbonate transport across cell membranes, which is essential for processes such as respiration and metabolic regulation. CA XII expression is high in normal tissues and has been implicated in various physiological and pathological conditions, including cancer, where expression can be altered. Understanding CA XII′s role in these processes can provide insights into potential therapeutic targets and biomarkers for disease.

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CA XII Antibody (D-2) References:

1. Human carbonic anhydrase XIV (CA14): cDNA cloning, mRNA expression, and mapping to chromosome 1.  |  Fujikawa-Adachi, K., et al. 1999. Genomics. 61: 74-81. PMID: 10512682
2. Purification and kinetic analysis of recombinant CA XII, a membrane carbonic anhydrase overexpressed in certain cancers.  |  Ulmasov, B., et al. 2000. Proc Natl Acad Sci U S A. 97: 14212-7. PMID: 11121027
3. Human carbonic anhydrase IV: cDNA cloning, sequence comparison, and expression in COS cell membranes.  |  Okuyama, T., et al. 1992. Proc Natl Acad Sci U S A. 89: 1315-9. PMID: 1311094
4. Differential in vitro inhibitory effects of anticancer drugs on tumor-associated carbonic anhydrase isozymes CA IX and CA XII.  |  Ozensoy Guler, O., et al. 2008. Methods Find Exp Clin Pharmacol. 30: 335-40. PMID: 18806891
5. Carbonic anhydrase II deficiency syndrome in a Belgian family is caused by a point mutation at an invariant histidine residue (107 His----Tyr): complete structure of the normal human CA II gene.  |  Venta, PJ., et al. 1991. Am J Hum Genet. 49: 1082-90. PMID: 1928091
6. The tumour-associated carbonic anhydrases CA II, CA IX and CA XII in a group of medulloblastomas and supratentorial primitive neuroectodermal tumours: an association of CA IX with poor prognosis.  |  Nordfors, K., et al. 2010. BMC Cancer. 10: 148. PMID: 20398423
7. o-Benzenedisulfonimido-sulfonamides are potent inhibitors of the tumor-associated carbonic anhydrase isoforms CA IX and CA XII.  |  Güzel-Akdemir, Ö., et al. 2013. Bioorg Med Chem. 21: 1386-91. PMID: 23352754
8. Fully Automated Production and Characterization of 64 Cu and Proof-of-Principle Small-Animal PET Imaging Using 64 Cu-Labelled CA XII Targeting 6A10 Fab.  |  Fiedler, L., et al. 2018. ChemMedChem. 13: 1230-1237. PMID: 29667369
9. Prognostic value of carbonic anhydrase XII (CA XII) overexpression in hepatocellular carcinoma.  |  Gu, XF., et al. 2019. Int J Clin Exp Pathol. 12: 2173-2183. PMID: 31934040
10. Novel benzenesulfonamide-thiouracil conjugates with a flexible N-ethyl acetamide linker as selective CA IX and CA XII inhibitors.  |  Abdel-Mohsen, HT., et al. 2023. Arch Pharm (Weinheim). 356: e2200434. PMID: 36372524
11. Human carbonic anhydrases and carbonic anhydrase deficiencies.  |  Sly, WS. and Hu, PY. 1995. Annu Rev Biochem. 64: 375-401. PMID: 7574487
12. Down-regulation of transmembrane carbonic anhydrases in renal cell carcinoma cell lines by wild-type von Hippel-Lindau transgenes.  |  Ivanov, SV., et al. 1998. Proc Natl Acad Sci U S A. 95: 12596-601. PMID: 9770531

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CA XII Antibody (D-2) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-CA XII Antibody (D-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CA XII Antibody (D-2) Specifications

Alternate Product Name: Carbonic anhydrase 12 (CA12)

Product Clone Name: D-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 241-354

Epitope Target: CA XII

Epitope Species: human

Product Reactivity/Detection: CA XII

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for CA XII Antibody (D-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred CA XII antibody is CA XII Antibody (A-3): sc-374313.