CA I Antibody (F-5): sc-393490

CA I Antibody (F-5) is a mouse monoclonal IgG2b kappa light chain antibody that detects CA I protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). CA I (F-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Carbonic anhydrases (CAs), also known as carbonate dehydratases, are a significant family of zinc metalloenzymes that play crucial roles in numerous physiological processes. CA I is particularly important due to involvement in the reversible hydration of carbon dioxide, which is essential for maintaining acid-base balance in the body. This enzyme facilitates the conversion of carbon dioxide to bicarbonate, a reaction vital for respiration and pH level regulation in various tissues. CA I is localized in the cytoplasm, participating in the formation of key biological fluids such as saliva and gastric acid, thereby influencing digestive processes. The CA1 gene, which encodes human CA I, is located on chromosome 8 and is part of a gene cluster that includes other carbonic anhydrase genes, highlighting the evolutionary significance and functional diversity of this enzyme family. Research has shown that while a severe deficiency of CA I does not lead to significant hematological or renal issues, proper function remains critical for overall metabolic homeostasis.

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CA I Antibody (F-5) References:

1. Immunohistochemistry of carbonic anhydrase isozymes (CA-I, II and III) in canine salivary glands: a distributional and comparative assessment.  |  Asari, M., et al. 2000. Anat Histol Embryol. 29: 9-12. PMID: 10820896
2. Carbonic anhydrase inhibitors: synthesis of sulfonamides incorporating 2,4,6-trisubstituted-pyridinium-ethylcarboxamido moieties possessing membrane-impermeability and in vivo selectivity for the membrane-bound (CA IV) versus the cytosolic (CA I and CA II) isozymes.  |  Supuran, CT., et al. 2000. J Enzyme Inhib. 15: 381-401. PMID: 10995069
3. Differential expression of cytoplasmic carbonic anhydrases, CA I and II, and membrane-associated isozymes, CA IX and XII, in normal mucosa of large intestine and in colorectal tumors.  |  Kivela, AJ., et al. 2001. Dig Dis Sci. 46: 2179-86. PMID: 11680594
4. Regional localization of carbonic anhydrase genes CA1 and CA3 on human chromosome 8.  |  Davis, MB., et al. 1987. Somat Cell Mol Genet. 13: 173-8. PMID: 3105094
5. Assignment of the gene determining human carbonic anhydrase, CAI, to chromosome 8.  |  Edwards, YH., et al. 1986. Ann Hum Genet. 50: 123-9. PMID: 3124707
6. The detection and differentiation of the products of the human carbonic anhydrase loci, CAI and CAII using fluorogenic substrates.  |  Hopkinson, DA., et al. 1974. Ann Hum Genet. 38: 155-62. PMID: 4219722
7. Functional diversity, conservation, and convergence in the evolution of the alpha-, beta-, and gamma-carbonic anhydrase gene families.  |  Hewett-Emmett, D. and Tashian, RE. 1996. Mol Phylogenet Evol. 5: 50-77. PMID: 8673298
8. Immunohistolocalization of carbonic anhydrase isozymes (CA-I, CA-II and CA-III) in bovine male reproductive tracts.  |  Ichihara, N., et al. 1997. Okajimas Folia Anat Jpn. 74: 193-8. PMID: 9446931

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CA I Antibody (F-5) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-CA I Antibody (F-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CA I Antibody (F-5) Specifications

Alternate Product Name: Carbonic anhydrase 1 (CA1)

Product Clone Name: F-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 45-64

Epitope Target: CA I (carbonic anhydrase)

Epitope Species: human

Product Reactivity/Detection: CA I

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for CA I Antibody (F-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin