BUB1 Antibody (14H5) recommended for detection of BUB1 of human origin by WB, IP, IF and ELISA

BUB1 Antibody (14H5): sc-47743

BUB1 Antibody (14H5) is rated 4.5 out of 5 by 4.
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Datasheets

    • BUB1 Antibody (14H5) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against full length recombinant human Bub1
    • recommended for detection of BUB1 of human origin by WB, IP, IF and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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What is the recommended starting dilution for Western blot for sc-47743: BUB1 (14H5) monoclonal antibody?

Asked by: jerojero
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 4 out of 5 by from Light bands seen in WB using HeLa and Nocodazole Light bands seen in WB using HeLa and Nocodazole treated HeLa whole cell lysates. -SCBT QC
Date published: 2015-06-11
Rated 5 out of 5 by from Nice band seen in WB using K Nice band seen in WB using K-562 whole cell lysate. -SCBT QC
Date published: 2015-05-03
Rated 4 out of 5 by from Worked well with transfected lysate by WB Worked well with transfected lysate by WB. -SCBT QC
Date published: 2015-04-13
Rated 5 out of 5 by from WB publishable data with human HEp WB publishable data with human HEp-2 and KB - SCBT Publication Review
Date published: 2013-04-27
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