BUB1 Antibody (B-3) recommended for detection of BUB1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

BUB1 Antibody (B-3): sc-365685

BUB1 Antibody (B-3) is rated 4.2 out of 5 by 6.
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Datasheets

    • Anti-BUB1 Antibody (B-3) is a mouse monoclonal IgG3 BUB1 antibody provided at 200 µg/ml
    • raised against amino acids 786-1085 mapping at the C-terminus of BUB1 of human origin
    • recommended for detection of BUB1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of BUB1 (B-3): sc-365685.
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Why does your datasheet say that the molecular weight of BUB1 is 150kDa but the Western blots show the protein being about 100kDa. What size band should I be looking for (Human protein)?

Asked by: tasutria
Thank you for your question. The listed molecular weight on the datasheet refers to a publication that cited the size for BUB1 as 150 kDa (PubMedID: 1142067), however published weights vary as low as 110 kDa for this protein (PubMedID: 10704439). The predicted weight based on sequence for BUB1 is ~122 kDa, and it undergoes post-translational modification including multiple phosphorylation sites, which changes it's observed weight on the gel. Our data falls within the observed range of 110-150 kDa. If you have any further questions please contact our Technical Services Department.
Answered by: Technical Service
Date published: 2017-03-01

I am using BUB1 (B-3): sc-365685 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Germaine
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from The antibody doesn't recognise BUB1. I did a siRNA experiment to check if the band that the antibody showed was Bub1 or not and the band didn't disappear. I double checked this with another antibody and the experiment worked properly so it has to be the antibody.
Date published: 2019-04-04
Rated 5 out of 5 by from Great antibody for IF! We received a sample and tested it in human colon carcinoma cells. It works really well in IF. Strong kinetochore signal only with PFA fixation!
Date published: 2017-11-09
Rated 5 out of 5 by from 该抗体对试验帮助很好 The specificity of this antibody is very well and good at my research。
Date published: 2017-09-20
Rated 5 out of 5 by from Versatile Antibody This antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC and ELISA. Strong, clear signal with little to no background. Beautiful tissue staining. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-01-23
Rated 4 out of 5 by from Strong bands seen in WB using HeLa and K Strong bands seen in WB using HeLa and K-562 whole cell lysates. -SCBT QC
Date published: 2015-04-16
Rated 5 out of 5 by from Exceptional immunoperoxidase cytoplasmic Exceptional immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human oral mucosa tissue. -SCBT QC
Date published: 2015-01-24
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