BTBD14A Antibody (A-7): sc-390650
- BTBD14A Antibody (A-7) is a mouse monoclonal IgG1 κ BTBD14A antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 479-512 within an internal region of BTBD14A of human origin
- BTBD14A Antibody (A-7) is recommended for detection of BTBD14A of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine and canine
- Anti-BTBD14A Antibody (A-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for BTBD14A Antibody (A-7) for WB and IHC(P) applications. These reagents are now offered in bundles with BTBD14A Antibody (A-7) (see ordering information below).
BTBD14A Antibody (A-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects BTBD14A of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). BTBD14A (A-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. BTBD14A, also known as BTB/POZ domain-containing protein 14A, consists of 587 amino acids and features a single BTB/POZ domain, which mediates both homomeric and heteromeric interactions among proteins. This domain plays a crucial role in transcriptional regulators involved in chromatin remodeling, significantly impacting gene expression regulation. BTBD14A interacts with SMRT/N-CoR-mSin3A histone deacetylase complex, essential for repressing and silencing gene transcription. Deletion of BTB/POZ domain, as observed in oncogenic PLZF-RAR chimera associated with promyelocytic leukemias, leads to loss of transcriptional repression, suggesting BTBD14A′s key role in regulating transcriptional activity.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
BTBD14A Antibody (A-7) References:
- Recruitment of SMRT/N-CoR-mSin3A-HDAC-repressing complexes is not a general mechanism for BTB/POZ transcriptional repressors: the case of HIC-1 and gammaFBP-B. | Deltour, S., et al. 1999. Proc Natl Acad Sci U S A. 96: 14831-6. PMID: 10611298
- Critical residues within the BTB domain of PLZF and Bcl-6 modulate interaction with corepressors. | Melnick, A., et al. 2002. Mol Cell Biol. 22: 1804-18. PMID: 11865059
- DNA sequence and analysis of human chromosome 9. | Humphray, SJ., et al. 2004. Nature. 429: 369-74. PMID: 15164053
- POZ for effect--POZ-ZF transcription factors in cancer and development. | Kelly, KF. and Daniel, JM. 2006. Trends Cell Biol. 16: 578-87. PMID: 16996269
- Histone deacetylase associated with mSin3A mediates repression by the acute promyelocytic leukemia-associated PLZF protein. | David, G., et al. 1998. Oncogene. 16: 2549-56. PMID: 9627120
- Components of the SMRT corepressor complex exhibit distinctive interactions with the POZ domain oncoproteins PLZF, PLZF-RARalpha, and BCL-6. | Wong, CW. and Privalsky, ML. 1998. J Biol Chem. 273: 27695-702. PMID: 9765306
- Crystal structure of the BTB domain from PLZF. | Ahmad, KF., et al. 1998. Proc Natl Acad Sci U S A. 95: 12123-8. PMID: 9770450
- The BCL-6 POZ domain and other POZ domains interact with the co-repressors N-CoR and SMRT. | Huynh, KD. and Bardwell, VJ. 1998. Oncogene. 17: 2473-84. PMID: 9824158
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BTBD14A Antibody (A-7) | sc-390650 | 200 µg/ml | $316.00 | |||
BTBD14A Antibody (A-7): m-IgG Fc BP-HRP Bundle | sc-530213 | 200 µg Ab; 10 µg BP | $354.00 | |||
BTBD14A Antibody (A-7): m-IgGκ BP-HRP Bundle | sc-523617 | 200 µg Ab, 40 µg BP | $354.00 | |||
BTBD14A Antibody (A-7): m-IgG1 BP-HRP Bundle | sc-543933 | 200 µg Ab; 20 µg BP | $354.00 | |||
BTBD14A Antibody (A-7) AC | sc-390650 AC | 500 µg/ml, 25% agarose | $416.00 | |||
BTBD14A Antibody (A-7) HRP | sc-390650 HRP | 200 µg/ml | $316.00 | |||
BTBD14A Antibody (A-7) FITC | sc-390650 FITC | 200 µg/ml | $330.00 | |||
BTBD14A Antibody (A-7) PE | sc-390650 PE | 200 µg/ml | $343.00 | |||
BTBD14A Antibody (A-7) Alexa Fluor® 488 | sc-390650 AF488 | 200 µg/ml | $357.00 | |||
BTBD14A Antibody (A-7) Alexa Fluor® 546 | sc-390650 AF546 | 200 µg/ml | $357.00 | |||
BTBD14A Antibody (A-7) Alexa Fluor® 594 | sc-390650 AF594 | 200 µg/ml | $357.00 | |||
BTBD14A Antibody (A-7) Alexa Fluor® 647 | sc-390650 AF647 | 200 µg/ml | $357.00 | |||
BTBD14A Antibody (A-7) Alexa Fluor® 680 | sc-390650 AF680 | 200 µg/ml | $357.00 | |||
BTBD14A Antibody (A-7) Alexa Fluor® 790 | sc-390650 AF790 | 200 µg/ml | $357.00 | |||
BTBD14A (A-7) Neutralizing Peptide | sc-390650 P | 100 µg/0.5 ml | $68.00 |