BRCA1 Antibody (D-9): sc-6954
- BRCA1 Antibody (D-9) is a mouse monoclonal IgG2a κ BRCA1 antibody, cited in 454 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 1843-1862 at the C-terminus of BRCA1 of human origin
- BRCA1 Antibody (D-9) is recommended for detection of BRCA1 of human origin by WB, IP, IF and ELISA
- Anti-BRCA1 Antibody (D-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for BRCA1 Antibody (D-9) for WB applications. These reagents are now offered in bundles with BRCA1 Antibody (D-9) (see ordering information below).
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BRCA1 Antibody (D-9) is a mouse monoclonal IgG2a kappa light chain antibody that detects BRCA1 of human origin by western blotting, immunoprecipitation, immunofluorescence, and enzyme-linked immunosorbent assay. BRCA1 (D-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. BRCA1, identified in 1990 and localized to chromosome 17q, plays a crucial role in maintaining genomic stability and is essential for DNA repair processes. BRCA1 mutations are linked to approximately 45% of families with a high incidence of breast cancer and at least 80% of families with increased rates of both early-onset breast cancer and ovarian cancer. Unlike BRCA2, which is located on chromosome 13q13.1, BRCA1 is a significant tumor suppressor that negatively regulates tumor growth, making BRCA1 vital in cancer prevention. BRCA1, consisting of 1863 amino acids, features a zinc finger domain in its amino terminal region, which is critical for interaction with other proteins involved in DNA repair and cell cycle regulation. BRCA1 (D-9) monoclonal antibody can provide valuable insights into mechanisms of breast and ovarian cancer, as well as broader implications of BRCA1 in tumor biology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
BRCA1 Antibody (D-9) References:
- BRCA1 physically and functionally interacts with ATF1. | Houvras, Y., et al. 2000. J Biol Chem. 275: 36230-7. PMID: 10945975
- BRCA1 cooperates with NUFIP and P-TEFb to activate transcription by RNA polymerase II. | Cabart, P., et al. 2004. Oncogene. 23: 5316-29. PMID: 15107825
- Familial breast-ovarian cancer locus on chromosome 17q12-q23. | Narod, SA., et al. 1991. Lancet. 338: 82-3. PMID: 1676470
- Linkage of early-onset familial breast cancer to chromosome 17q21. | Hall, JM., et al. 1990. Science. 250: 1684-9. PMID: 2270482
- A strong candidate for the breast and ovarian cancer susceptibility gene BRCA1. | Miki, Y., et al. 1994. Science. 266: 66-71. PMID: 7545954
- BRCA1 mutations in primary breast and ovarian carcinomas. | Futreal, PA., et al. 1994. Science. 266: 120-2. PMID: 7939630
- Breast cancer gene offers surprises. | Nowak, R. 1994. Science. 265: 1796-9. PMID: 8091205
- Localization of a breast cancer susceptibility gene, BRCA2, to chromosome 13q12-13. | Wooster, R., et al. 1994. Science. 265: 2088-90. PMID: 8091231
- Nuclear redistribution of BRCA1 during viral infection. | Maul, GG., et al. 1998. Cell Growth Differ. 9: 743-55. PMID: 9751118
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BRCA1 Antibody (D-9) | sc-6954 | 200 µg/ml | $322.00 | |||
BRCA1 Antibody (D-9): m-IgG Fc BP-HRP Bundle | sc-528169 | 200 µg Ab; 10 µg BP | $361.00 | |||
BRCA1 Antibody (D-9): m-IgGκ BP-HRP Bundle | sc-520481 | 200 µg Ab, 40 µg BP | $361.00 | |||
BRCA1 Antibody (D-9): m-IgG2a BP-HRP Bundle | sc-547036 | 200 µg Ab; 10 µg BP | $361.00 | |||
BRCA1 Antibody (D-9) AC | sc-6954 AC | 500 µg/ml, 25% agarose | $424.00 | |||
BRCA1 Antibody (D-9) HRP | sc-6954 HRP | 200 µg/ml | $322.00 | |||
BRCA1 Antibody (D-9) FITC | sc-6954 FITC | 200 µg/ml | $336.00 | |||
BRCA1 Antibody (D-9) PE | sc-6954 PE | 200 µg/ml | $349.00 | |||
BRCA1 Antibody (D-9) Alexa Fluor® 488 | sc-6954 AF488 | 200 µg/ml | $364.00 | |||
BRCA1 Antibody (D-9) Alexa Fluor® 546 | sc-6954 AF546 | 200 µg/ml | $364.00 | |||
BRCA1 Antibody (D-9) Alexa Fluor® 594 | sc-6954 AF594 | 200 µg/ml | $364.00 | |||
BRCA1 Antibody (D-9) Alexa Fluor® 647 | sc-6954 AF647 | 200 µg/ml | $364.00 | |||
BRCA1 Antibody (D-9) Alexa Fluor® 680 | sc-6954 AF680 | 200 µg/ml | $364.00 | |||
BRCA1 Antibody (D-9) Alexa Fluor® 790 | sc-6954 AF790 | 200 µg/ml | $364.00 | |||
BRCA1 (D-9) Neutralizing Peptide | sc-6954 P | 100 µg/0.5 ml | $69.00 |