Date published: 2025-10-15
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BRCA1 Antibody (D-9) - Immunofluorescence - Image 323594
BRCA1 Antibody (D-9): sc-6954. Immunofluorescence staining of formalin-fixed, UVA laser-microirradiated U-2 OS cells showing nuclear staining of cells with DNA damage. Kindly provided by Yang Xiang, Ph.D., Division of Newborn Medicine, Boston Childrens Hospital, Cell Biology Department, Harvard Medical Schoo
BRCA1 Antibody (D-9): sc-6954. Immunofluorescence staining of formalin-fixed, UVA laser-microirradiated U-2 OS cells showing nuclear staining of cells with DNA damage. Kindly provided by Yang Xiang, Ph.D., Division of Newborn Medicine, Boston Childrens Hospital, Cell Biology Department, Harvard Medical Schoo

BRCA1 Antibody (D-9): sc-6954

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Datasheets
  • BRCA1 Antibody (D-9) is a mouse monoclonal IgG2a κ BRCA1 antibody, cited in 454 publications, provided at 200 µg/ml
  • specific for an epitope mapping between amino acids 1843-1862 at the C-terminus of BRCA1 of human origin
  • BRCA1 Antibody (D-9) is recommended for detection of BRCA1 of human origin by WB, IP, IF and ELISA
  • Anti-BRCA1 Antibody (D-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for BRCA1 Antibody (D-9) for WB applications. These reagents are now offered in bundles with BRCA1 Antibody (D-9) (see ordering information below).

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SEE ALSO...

Additional BRCA Antibodies including BRCA2

BRCA1 Antibody (D-9) is a mouse monoclonal IgG2a kappa light chain antibody that detects BRCA1 of human origin by western blotting, immunoprecipitation, immunofluorescence, and enzyme-linked immunosorbent assay. BRCA1 (D-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. BRCA1, identified in 1990 and localized to chromosome 17q, plays a crucial role in maintaining genomic stability and is essential for DNA repair processes. BRCA1 mutations are linked to approximately 45% of families with a high incidence of breast cancer and at least 80% of families with increased rates of both early-onset breast cancer and ovarian cancer. Unlike BRCA2, which is located on chromosome 13q13.1, BRCA1 is a significant tumor suppressor that negatively regulates tumor growth, making BRCA1 vital in cancer prevention. BRCA1, consisting of 1863 amino acids, features a zinc finger domain in its amino terminal region, which is critical for interaction with other proteins involved in DNA repair and cell cycle regulation. BRCA1 (D-9) monoclonal antibody can provide valuable insights into mechanisms of breast and ovarian cancer, as well as broader implications of BRCA1 in tumor biology.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

BRCA1 Antibody (D-9) References:

  1. BRCA1 physically and functionally interacts with ATF1.  |  Houvras, Y., et al. 2000. J Biol Chem. 275: 36230-7. PMID: 10945975
  2. BRCA1 cooperates with NUFIP and P-TEFb to activate transcription by RNA polymerase II.  |  Cabart, P., et al. 2004. Oncogene. 23: 5316-29. PMID: 15107825
  3. Familial breast-ovarian cancer locus on chromosome 17q12-q23.  |  Narod, SA., et al. 1991. Lancet. 338: 82-3. PMID: 1676470
  4. Linkage of early-onset familial breast cancer to chromosome 17q21.  |  Hall, JM., et al. 1990. Science. 250: 1684-9. PMID: 2270482
  5. A strong candidate for the breast and ovarian cancer susceptibility gene BRCA1.  |  Miki, Y., et al. 1994. Science. 266: 66-71. PMID: 7545954
  6. BRCA1 mutations in primary breast and ovarian carcinomas.  |  Futreal, PA., et al. 1994. Science. 266: 120-2. PMID: 7939630
  7. Breast cancer gene offers surprises.  |  Nowak, R. 1994. Science. 265: 1796-9. PMID: 8091205
  8. Localization of a breast cancer susceptibility gene, BRCA2, to chromosome 13q12-13.  |  Wooster, R., et al. 1994. Science. 265: 2088-90. PMID: 8091231
  9. Nuclear redistribution of BRCA1 during viral infection.  |  Maul, GG., et al. 1998. Cell Growth Differ. 9: 743-55. PMID: 9751118

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

BRCA1 Antibody (D-9)

sc-6954
200 µg/ml
$316.00

BRCA1 Antibody (D-9): m-IgG Fc BP-HRP Bundle

sc-528169
200 µg Ab; 10 µg BP
$354.00

BRCA1 Antibody (D-9): m-IgGκ BP-HRP Bundle

sc-520481
200 µg Ab, 40 µg BP
$354.00

BRCA1 Antibody (D-9): m-IgG2a BP-HRP Bundle

sc-547036
200 µg Ab; 10 µg BP
$354.00

BRCA1 Antibody (D-9) AC

sc-6954 AC
500 µg/ml, 25% agarose
$416.00

BRCA1 Antibody (D-9) HRP

sc-6954 HRP
200 µg/ml
$316.00

BRCA1 Antibody (D-9) FITC

sc-6954 FITC
200 µg/ml
$330.00

BRCA1 Antibody (D-9) PE

sc-6954 PE
200 µg/ml
$343.00

BRCA1 Antibody (D-9) Alexa Fluor® 488

sc-6954 AF488
200 µg/ml
$357.00

BRCA1 Antibody (D-9) Alexa Fluor® 546

sc-6954 AF546
200 µg/ml
$357.00

BRCA1 Antibody (D-9) Alexa Fluor® 594

sc-6954 AF594
200 µg/ml
$357.00

BRCA1 Antibody (D-9) Alexa Fluor® 647

sc-6954 AF647
200 µg/ml
$357.00

BRCA1 Antibody (D-9) Alexa Fluor® 680

sc-6954 AF680
200 µg/ml
$357.00

BRCA1 Antibody (D-9) Alexa Fluor® 790

sc-6954 AF790
200 µg/ml
$357.00

BRCA1 (D-9) Neutralizing Peptide

sc-6954 P
100 µg/0.5 ml
$68.00

3–8% Tris-acetate gel로 HAP-1 cell에서 WB을 진행. Ponceau 결과에서 단백질은 잘 transfer되었고, actin 역시 정상적으로 발현이 확인되었습니다. 그러나 target인 BRCA1은 검출되지 않았습니다. 현재 사용 중인 transfer 조건은 400 mA, 90분 (wet transfer)이며, BRCA1이 고분자 단백질이다 보니 transfer 조건을 건드려야할 것 같은데, 조언해주실 부분 있을까요?

Asked by: WaterD
Thank you for your question. Please contact our Technical Service Department for more information. Our Asian Technical Service team is available at asia@scbio.cn or by live chat on our website.
Answered by: Technical Service SH
Date published: 2025-08-11

Hello, is this antibody sequence available for engineering purposes? Or, how do I go about collaborating with SC to get the proprietary sequence for academic research?

Asked by: Brant Gracia
The sequences are proprietary for our antibodies.
Answered by: Technical Service
Date published: 2024-10-11

Can the antibody be used at 37 oC for 1 h for western blot?

Asked by: Elisabet Ognedal
Thank you for your question. We do not recommend using the antibody at 37 C. Please see our protocol for recommendations. https://www.scbt.com/resources/protocols/western-immuno-blotting
Answered by: BlakeJ
Date published: 2024-09-27

I have tried an aliquote of sc-6954 (Lot-J0622), borrowed from a collegue, and I have got no signal at all in western blot. I wanted this ab because it has lots of citations. Could I get a sample of a different Lot to test it before buying it? Thanks.

Asked by: Israel
Thank you for your question. Please contact our technical service team directly.
Answered by: Tech Support Europe
Date published: 2024-09-03

What Is the recommended blocking buffer for this antibody(sc-6954)? We have tried LI-COR’s PBS based blocking buffer and 5% BSA in PBST, but have not detected the expected bands, despite detection of phospho-Brca1. We are using several Ewing sarcoma lines

Asked by: Hortonj
Thank you for your question. We recommend blocking in 5% milk with TBS-T, or our recommended UltraCruz blocking reagent, sc-516214. Please contact Technical Service if you need any further assistance with this antibody!
Answered by: Tech Service
Date published: 2019-01-14

I am saneesh Babu Padmasaras working as a postdoctoral fellow in Department of Biochemsitry and molecular biology, I would like to get a free sample 10 µg sample of BRCA1 Antibody (D-9): sc-6954 , so kindly send me the antibody

Asked by: Saneesh
Thank you for your question. Please contact Technical Service for information on how to obtain a sample.
Answered by: Technical Support
Date published: 2018-12-14

如何联系你们申请试用装？

Asked by: 张晨aaa
感谢您的提问。关于试用装的申请事宜，欢迎通过：电话(+86) 021-60936350、邮箱asia@scbio.cn、或选择网页上Live Chat中的“中文支持”，联系我们的技术支持部门。 Thank you for your inquiry. For sample request, please contact our Asian Technical Service team at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Technical Support 15
Date published: 2018-02-11

Can BRCA1 Antibody (D-9) use for immunoprecipitation assey in human breast cancer cell lines? We are looking for papers.

Asked by: taro12345678
Thank you for your question. Yes, this mouse monoclonal, BRCA1 Antibody (D-9): sc-6954 can be used for immunoprecipitation (IP) on human cell lines. We are aware of a couple of publications citing use of the antibody for IP in human breast cancer cell lines. Please refer to PMID's: 27454289 and 25480944.
Answered by: Technical Support
Date published: 2017-06-26
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Rated 1 out of 5 by from Completely Blank BlotSC-6954, Lot# H1122, does not detect BRCA1 on western blot, even with 60µg whole cell extract from human HCT116 cells, 3-8% TA gel, 20% EtOH equilibration, 10' x 25V iblot transfer, and 1:100 O/N incubation. Positive control worked fine.
Date published: 2023-03-02
Rated 4 out of 5 by from Good for WBBy Western Blot (1:500 dilution), I can detect a clear band at the expected molecular weight.
Date published: 2022-11-07
Rated 1 out of 5 by from The antibody doesn't workWe just bought a new antibody to test by western blot. The wb is completely blank. other antibodies probing for the other protein works well (we do wb everyday). This antibody used to work during my postdoc research. Perhaps there's a change in production.
Date published: 2022-01-12
Rated 5 out of 5 by from Good antibody for western blottingI used this antibody for western blotting (1:100 dilution). A specific band was detected in cancer cell line as compared with BRCA1 KO cells.
Date published: 2021-12-23
Rated 5 out of 5 by from Works very wellWorks very well by immunofluorescence on cells (1:200 dilution). By western blot (1:1000 dilution) i can see a clear band with nuclear/cytoplasmic fractionation
Date published: 2021-11-26
Rated 5 out of 5 by from Great antibody for both IF and WBI have been using this anti-BRCA1 antibody for both IF and WB (1:100 dilution) for over a year now and couldn't be happier! Very specific (verified by knock-down in our lab) and good signal even with low protein expression.
Date published: 2021-01-04
Rated 1 out of 5 by from Failed to stain pachytene spermatocytesNo signal at all at 1:50, 1:100, 1:200 whatsoever. We tried goat and donkey serum - no difference. There were good reviews for the previous Ab C-20. C-20 was rabbite polyclonal and this one is mouse monoclonal, so I guess it skips a lot of signal. D-9 is probably good just for biochemistry. Example is the most recent paper that cited D-9 - in it D9 was used just for pull-downs and WB, but the cytology was done by C-20.
Date published: 2019-05-23
Rated 5 out of 5 by from 应该还不错你好，我在半年前买了BRCA1这个抗体，可能是我转膜时间不太对，跑了两次没有条带，我想请问一下，这种大分子的蛋白转条件应该如何把握，谢谢！
Date published: 2018-10-09
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BRCA1 Antibody (D-9) is rated 4.2 out of 5 by 53.
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