brachyury Antibody (A-4): sc-374321

brachyury Antibody (A-4) is a mouse monoclonal IgG2b kappa light chain antibody that detects brachyury in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). brachyury (A-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. brachyury, a member of the T-box gene family, encodes a transcription factor crucial for early vertebrate development, particularly in notochord and posterior mesoderm formation. The T-box gene family, which includes over 20 members identified in both invertebrates and vertebrates, is characterized by a unique DNA-binding domain known as the T-domain. This highly conserved motif is essential for the transcriptional activation functions of Tbx proteins, with brachyury serving as a prototypical example. Mutations in Tbx genes, including brachyury, have been linked to various human diseases, highlighting the importance of understanding their functional mechanisms. The role of brachyury in embryonic development is underscored by the observation that mutant embryos lacking the T gene exhibit deficiencies in notochord differentiation and posterior mesoderm formation, although they can still develop anterior mesoderm.

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brachyury Antibody (A-4) References:

1. Brachyury is a target gene of the Wnt/beta-catenin signaling pathway.  |  Arnold, SJ., et al. 2000. Mech Dev. 91: 249-58. PMID: 10704849
2. Brachyury null mutant-induced defects in juvenile ascidian endodermal organs.  |  Chiba, S., et al. 2009. Development. 136: 35-9. PMID: 19019990
3. Brachyury and related Tbx proteins interact with the Mixl1 homeodomain protein and negatively regulate Mixl1 transcriptional activity.  |  Pereira, LA., et al. 2011. PLoS One. 6: e28394. PMID: 22164283
4. Cancer vaccines targeting the epithelial-mesenchymal transition: tissue distribution of brachyury and other drivers of the mesenchymal-like phenotype of carcinomas.  |  Hamilton, DH., et al. 2012. Semin Oncol. 39: 358-66. PMID: 22595058
5. Brachyury regulates proliferation of cancer cells via a p27Kip1-dependent pathway.  |  Jezkova, J., et al. 2014. Oncotarget. 5: 3813-22. PMID: 25003467
6. Brachyury identifies a class of enteroendocrine cells in normal human intestinal crypts and colorectal cancer.  |  Jezkova, J., et al. 2016. Oncotarget. 7: 11478-86. PMID: 26862851
7. Brachyury, a vaccine target, is overexpressed in triple-negative breast cancer.  |  Hamilton, DH., et al. 2016. Endocr Relat Cancer. 23: 783-796. PMID: 27580659
8. Brachyury promotes tamoxifen resistance in breast cancer by targeting SIRT1.  |  Li, K., et al. 2016. Biomed Pharmacother. 84: 28-33. PMID: 27621036
9. Brachyury oncogene is a prognostic factor in high-risk testicular germ cell tumors.  |  Pinto, F., et al. 2018. Andrology. 6: 597-604. PMID: 29749711
10. Generation of Brachyury-mCherry knock-in reporter human pluripotent stem cell line (SNUe003-A-2) using CRISPR/CAS9 nuclease.  |  Jung, J., et al. 2021. Stem Cell Res. 53: 102321. PMID: 33878708

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-brachyury Antibody (A-4) has 18 citations in a variety of scientific publications.

Primary antibodies like Anti-brachyury Antibody (A-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

brachyury Antibody (A-4) Specifications

Alternate Product Name: brachyury

Product Clone Name: A-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 226-43

Epitope Target: brachyury

Epitope Species: human

Product Reactivity/Detection: brachyury

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 18 citations in various scientific publications

Available Conjugates/Forms for brachyury Antibody (A-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent