BPI Antibody (H-10): sc-514212
- BPI Antibody (H-10) is a mouse monoclonal IgG2a κ BPI antibody, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 229-253 to the elastase cleavage site of BPI of human origin
- BPI Antibody (H-10) is recommended for detection of BPI of human origin by WB, IP, IF and ELISA
- Anti-BPI Antibody (H-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for BPI Antibody (H-10) for WB applications. These reagents are now offered in bundles with BPI Antibody (H-10) (see ordering information below).
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BPI Antibody (H-10) is a mouse monoclonal IgG2a kappa light chain antibody that detects BPI protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-BPI antibody (H-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The bactericidal permeability increasing protein (BPI) plays a crucial role in the immune response by acting as an antibacterial and endotoxin-neutralizing agent, primarily found in the granules of polymorphonuclear leukocytes, such as neutrophils. The human BPI gene, which spans 31.5 kb and is located on chromosome 20q11.23-q12, consists of 15 exons and encodes a 456 amino acid protein. BPI is strategically localized to the cell surface of epithelial cells, which serve as the first line of defense against bacterial invasion. Such localization enables BPI to block endotoxin-mediated signaling, thereby safeguarding mucosal surfaces from the harmful effects of gram-negative bacteria and their endotoxins. BPI is part of a functionally related protein family that includes lipopolysaccharide binding protein, phospholipid transfer protein, and cholesteryl ester transfer protein, highlighting BPI′s importance in the broader context of immune defense mechanisms.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
BPI Antibody (H-10) References:
- Lipid mediator-induced expression of bactericidal/ permeability-increasing protein (BPI) in human mucosal epithelia. | Canny, G., et al. 2002. Proc Natl Acad Sci U S A. 99: 3902-7. PMID: 11891303
- Structure and function of lipopolysaccharide binding protein. | Schumann, RR., et al. 1990. Science. 249: 1429-31. PMID: 2402637
- Cloning of the cDNA of a human neutrophil bactericidal protein. Structural and functional correlations. | Gray, PW., et al. 1989. J Biol Chem. 264: 9505-9. PMID: 2722846
- A 25-kDa NH2-terminal fragment carries all the antibacterial activities of the human neutrophil 60-kDa bactericidal/permeability-increasing protein. | Ooi, CE., et al. 1987. J Biol Chem. 262: 14891-4. PMID: 3667613
- The genes for the lipopolysaccharide binding protein (LBP) and the bactericidal permeability increasing protein (BPI) are encoded in the same region of human chromosome 20. | Gray, PW., et al. 1993. Genomics. 15: 188-90. PMID: 8432532
- Crystal structure of human BPI and two bound phospholipids at 2.4 angstrom resolution. | Beamer, LJ., et al. 1997. Science. 276: 1861-4. PMID: 9188532
- The genomic organization of the genes for human lipopolysaccharide binding protein (LBP) and bactericidal permeability increasing protein (BPI) is highly conserved. | Hubacek, JA., et al. 1997. Biochem Biophys Res Commun. 236: 427-30. PMID: 9240454
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BPI Antibody (H-10) | sc-514212 | 200 µg/ml | $322.00 | |||
BPI Antibody (H-10): m-IgG Fc BP-HRP Bundle | sc-531066 | 200 µg Ab; 10 µg BP | $361.00 | |||
BPI Antibody (H-10): m-IgGκ BP-HRP Bundle | sc-524649 | 200 µg Ab, 40 µg BP | $361.00 | |||
BPI Antibody (H-10): m-IgG2a BP-HRP Bundle | sc-547677 | 200 µg Ab; 10 µg BP | $361.00 | |||
BPI Antibody (H-10) AC | sc-514212 AC | 500 µg/ml, 25% agarose | $424.00 | |||
BPI Antibody (H-10) HRP | sc-514212 HRP | 200 µg/ml | $322.00 | |||
BPI Antibody (H-10) FITC | sc-514212 FITC | 200 µg/ml | $336.00 | |||
BPI Antibody (H-10) PE | sc-514212 PE | 200 µg/ml | $349.00 | |||
BPI Antibody (H-10) Alexa Fluor® 488 | sc-514212 AF488 | 200 µg/ml | $364.00 | |||
BPI Antibody (H-10) Alexa Fluor® 546 | sc-514212 AF546 | 200 µg/ml | $364.00 | |||
BPI Antibody (H-10) Alexa Fluor® 594 | sc-514212 AF594 | 200 µg/ml | $364.00 | |||
BPI Antibody (H-10) Alexa Fluor® 647 | sc-514212 AF647 | 200 µg/ml | $364.00 | |||
BPI Antibody (H-10) Alexa Fluor® 680 | sc-514212 AF680 | 200 µg/ml | $364.00 | |||
BPI Antibody (H-10) Alexa Fluor® 790 | sc-514212 AF790 | 200 µg/ml | $364.00 | |||
BPI (H-10) Neutralizing Peptide | sc-514212 P | 100 µg/0.5 ml | $69.00 |