BLM Antibody (B-4): sc-365753

BLM Antibody (B-4) is a mouse monoclonal IgG2b kappa light chain antibody that detects BLM protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). BLM (B-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. BLM protein plays a crucial role in maintaining genomic stability by unwinding DNA during replication and repair processes, which is vital for preventing mutations that can lead to cancer. Mutations in BLM gene are associated with Bloom′s syndrome, an autosomal recessive disorder characterized by growth deficiencies, sun sensitivity, immunodeficiency, and a significantly increased risk of various cancers. One of the hallmark features of Bloom′s syndrome is the elevated frequency of sister chromatid exchange, which is linked to the impaired function of BLM protein in unwinding G4 DNA structures. BLM is predominantly located in the nucleus, where ATPase activity is activated by both single- and double-stranded DNA, highlighting an essential role in DNA metabolism. Additionally, the homologous yeast protein SGS1, which shares similarities with BLM, has been shown to cause mitotic hyperrecombination when mutated, further underscoring the importance of BLM in maintaining genomic integrity.

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BLM Antibody (B-4) References:

1. Replication protein A physically interacts with the Bloom's syndrome protein and stimulates its helicase activity.  |  Brosh, RM., et al. 2000. J Biol Chem. 275: 23500-8. PMID: 10825162
2. BLM, the Bloom's syndrome protein, varies during the cell cycle in its amount, distribution, and co-localization with other nuclear proteins.  |  Sanz, MM., et al. 2000. Cytogenet Cell Genet. 91: 217-23. PMID: 11173860
3. Cleavage of BLM and sensitivity of Bloom's syndrome cells to hydroxurea and UV-C radiation.  |  Ababou, M., et al. 2002. Cell Cycle. 1: 262-6. PMID: 12429945
4. Stimulation of flap endonuclease-1 by the Bloom's syndrome protein.  |  Sharma, S., et al. 2004. J Biol Chem. 279: 9847-56. PMID: 14688284
5. Syndrome-causing mutations of the BLM gene in persons in the Bloom's Syndrome Registry.  |  German, J., et al. 2007. Hum Mutat. 28: 743-53. PMID: 17407155
6. Cellular defects caused by hypomorphic variants of the Bloom syndrome helicase gene BLM.  |  Shastri, VM. and Schmidt, KH. 2016. Mol Genet Genomic Med. 4: 106-19. PMID: 26788541
7. The Bloom's syndrome gene product is homologous to RecQ helicases.  |  Ellis, NA., et al. 1995. Cell. 83: 655-66. PMID: 7585968
8. Bloom syndrome: is the gene mapped to the point?  |  Bamezai, R. 1996. Indian J Exp Biol. 34: 298-301. PMID: 8698416
9. SGS1, a homologue of the Bloom's and Werner's syndrome genes, is required for maintenance of genome stability in Saccharomyces cerevisiae.  |  Watt, PM., et al. 1996. Genetics. 144: 935-45. PMID: 8913739
10. The Bloom's syndrome gene product is a 3'-5' DNA helicase.  |  Karow, JK., et al. 1997. J Biol Chem. 272: 30611-4. PMID: 9388193
11. BLM (the causative gene of Bloom syndrome) protein translocation into the nucleus by a nuclear localization signal.  |  Kaneko, H., et al. 1997. Biochem Biophys Res Commun. 240: 348-53. PMID: 9388480
12. The Bloom's syndrome helicase unwinds G4 DNA.  |  Sun, H., et al. 1998. J Biol Chem. 273: 27587-92. PMID: 9765292

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-BLM Antibody (B-4) has 17 citations in a variety of scientific publications.

Primary antibodies like Anti-BLM Antibody (B-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

BLM Antibody (B-4) Specifications

Alternate Product Name: Bloom syndrome protein

Product Clone Name: B-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1118-1417

Epitope Target: BLM

Epitope Species: human

Product Reactivity/Detection: BLM

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 17 citations in various scientific publications

Available Conjugates/Forms for BLM Antibody (B-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin