β-casein Antibody (F-4): sc-166684

β-casein Antibody (F-4) is a mouse monoclonal IgG2b antibody that detects β-casein in mouse samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). β-casein plays a vital role in the nutritional development of newborn mammals, as one of the primary proteins found in mammalian milk, particularly in human milk where β-casein exists alongside k-casein. The unique structure of β-casein, characterized by highly phosphorylated sites, is crucial for calcium-binding capacity, which enhances the digestibility of casein micelles in colostrum, thereby providing essential nutrients to neonates during their critical early days of life. The regulation of β-casein gene expression involves various transcription factors such as Stat5 and C/EBPβ, which bind to specific sites in the gene′s enhancer region, allowing for the integration of signals from lactogenic hormones like prolactin and insulin. Additionally, NFκB acts as a negative regulator during pregnancy, highlighting the intricate balance of factors that control β-casein production, ensuring optimal protein amounts when needed most. β-casein Antibody (F-4) serves as an invaluable tool for researchers investigating milk protein dynamics and their implications for neonatal health.

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β-casein Antibody (F-4) References:

1. Plasmin cleavage of human beta-casein.  |  Azuma, N. and Yamauchi, K. 1992. Biosci Biotechnol Biochem. 56: 1140-1. PMID: 1369431
2. Pressure-aided proteolysis of beta-casein.  |  Bruins, ME., et al. 2009. J Agric Food Chem. 57: 5529-34. PMID: 19459710
3. Binding mechanism and antioxidant capacity of selected phenolic acid - β-casein complexes.  |  Li, T., et al. 2020. Food Res Int. 129: 108802. PMID: 32036926
4. A human β-casein-derived peptide BCCY-1 modulates the innate immune response.  |  Cai, J., et al. 2021. Food Chem. 348: 129111. PMID: 33516994
5. Temperature-dependent dissociation of human micellar β-casein: Implications of its phosphorylation degrees and casein micelle structures.  |  Yang, T., et al. 2021. Food Chem. 376: 131935. PMID: 34968916
6. Bovine milk with variant β-casein types on immunological mediated intestinal changes and gut health of mice.  |  Liu, B., et al. 2022. Front Nutr. 9: 970685. PMID: 36245506
7. The cysteine residue in beta-lactoglobulin reacts with oxidized tyrosine residues in beta-casein to give casein-lactoglobulin dimers.  |  Doblas, L., et al. 2023. Arch Biochem Biophys. 733: 109482. PMID: 36457258
8. Modification of a previously patented method to unequivocally score A2-like and A1-like bovine β-casein variants.  |  Raschia, MA., et al. 2023. MethodsX. 10: 102183. PMID: 37424753
9. Immobilized enzyme microreactors for analysis of tryptic peptides in β-casein and β-lactoglobulin.  |  Rodzik, A., et al. 2023. Sci Rep. 13: 16551. PMID: 37783762
10. The Immunomodulatory Effects of A2 β-Casein on Immunosuppressed Mice by Regulating Immune Responses and the Gut Microbiota.  |  Li, X., et al. 2024. Nutrients. 16: PMID: 38398845

The preferred β-casein antibody is β-casein Antibody (F20.14): sc-53189.