BChE Antibody (D-5): sc-377403

BChE Antibody (D-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects butyrylcholine esterase (BChE) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-BChE antibody (D-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Butyrylcholine esterase, also known as acylcholine acylhydrolase or pseudocholinesterase, is predominantly synthesized in the liver and hydrolyzes acylcholine into choline and carboxylate, playing a significant role in neurotransmission and detoxification of harmful substances. BChE is present in most cells except erythrocytes and exhibits esterase and aryl acylamidase activities, which maintain cholinergic signaling and modulate effects of organophosphate esters. BChE can form a homotetramer structure composed of two dimers linked by a disulfide bond, essential for enzymatic function and stability. Deficiencies in BChE are linked to hypocholinesterasemia, a condition leading to various neurological issues. Additionally, BChE inhibition affects organophosphate toxicity in the respiratory system, suggesting potential therapeutic applications in cases of intoxication with substances such as cocaine.

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BChE Antibody (D-5) References:

1. Effects of acetylcholinesterase and butyrylcholinesterase inhibition on breathing in mice adapted or not to reduced acetylcholinesterase.  |  Boudinot, E., et al. 2005. Pharmacol Biochem Behav. 80: 53-61. PMID: 15652380
2. Candidate gene association studies of genes involved in neuronal cholinergic transmission in Alzheimer's disease suggests choline acetyltransferase as a candidate deserving further study.  |  Cook, LJ., et al. 2005. Am J Med Genet B Neuropsychiatr Genet. 132B: 5-8. PMID: 15690550
3. Inherited and acquired interactions between ACHE and PON1 polymorphisms modulate plasma acetylcholinesterase and paraoxonase activities.  |  Bryk, B., et al. 2005. J Neurochem. 92: 1216-27. PMID: 15715671
4. Novel inhibitors of acetyl- and butyrylcholinesterase derived from the alkaloids dehydroevodiamine and rutaecarpine.  |  Decker, M. 2005. Eur J Med Chem. 40: 305-13. PMID: 15725500
5. Cholinesterase activity in human pulmonary arteries and veins: correlation with mRNA levels.  |  Kotelevets, L., et al. 2005. Life Sci. 76: 2211-20. PMID: 15733936
6. Four new mutations in the BCHE gene of human butyrylcholinesterase in a Brazilian blood donor sample.  |  Souza, RL., et al. 2005. Mol Genet Metab. 84: 349-53. PMID: 15781196
7. Interaction between mivacurium and pancuronium: impact of the order of administration.  |  Motamed, C., et al. 2005. Eur J Clin Pharmacol. 61: 175-7. PMID: 15824913
8. Reaction kinetics of biotinylated organophosphorus toxicant, FP-biotin, with human acetylcholinesterase and human butyrylcholinesterase.  |  Schopfer, LM., et al. 2005. Chem Res Toxicol. 18: 747-54. PMID: 15833035
9. Isolation of a galactose-free 20-kDa fragment exhibiting butyrylcholine esterase and aryl acylamidase activity from human serum butyrylcholine esterase by limited alpha-chymotrypsin digestion.  |  Rao, RV. and Balasubramanian, AS. 1989. Eur J Biochem. 179: 639-44. PMID: 2646120
10. Effect of rivastigmine on plasma butyrylcholine esterase activity and plasma ghrelin levels in patients with dementia in Alzheimer's disease.  |  Kuroda, A., et al. 2018. Geriatr Gerontol Int. 18: 886-891. PMID: 29441654
11. Extending the lore of curcumin as dipteran Butyrylcholine esterase (BChE) inhibitor: A holistic molecular interplay assessment.  |  Rao, P., et al. 2022. PLoS One. 17: e0269036. PMID: 35617284
12. Progressions of the correlation between lipid metabolism and immune infiltration characteristics in gastric cancer and identification of BCHE as a potential biomarker.  |  Wang, S., et al. 2024. Front Immunol. 15: 1327565. PMID: 38357546

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-BChE Antibody (D-5) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-BChE Antibody (D-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

BChE Antibody (D-5) Specifications

Alternate Product Name: BChE

Product Clone Name: D-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 85-119

Epitope Target: BChE

Epitope Species: human

Product Reactivity/Detection: mature BChE and Cholinesterase precursor

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for BChE Antibody (D-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin