BAP31 Antibody (D-6): sc-393810

BAP31 Antibody (D-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects BAP31 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). BAP31 (D-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. BAP31, a human Bcl-2-interacting protein, is an integral membrane protein located in the endoplasmic reticulum, where BAP31 plays a crucial role in apoptosis regulation. BAP31 is part of a complex that connects apoptosis-initiating caspases, such as procaspase-8, with anti-apoptotic regulators like Bcl-2 or Bcl-XL, thereby influencing cell survival and death pathways. The cytosolic domain of BAP31 contains two identical caspase recognition sites that are cleaved preferentially by initiator caspases, leading to the generation of a p20 fragment during apoptosis. This fragment remains integrated in the membrane and, when ectopically expressed, acts as a potent inducer of cell death. BAP31 cleavage is vital for the manifestation of cytoplasmic apoptotic events, including membrane fragmentation and the interplay between mitochondria and the endoplasmic reticulum during Fas-mediated apoptosis. BAP31 gene is ubiquitously expressed across murine tissues and is located on the X chromosome in both mouse and human, highlighting BAP31′s significance in cellular processes and potential implications in disease states.

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BAP31 Antibody (D-6) References:

1. Caspase-resistant BAP31 inhibits fas-mediated apoptotic membrane fragmentation and release of cytochrome c from mitochondria.  |  Nguyen, M., et al. 2000. Mol Cell Biol. 20: 6731-40. PMID: 10958671
2. Interaction between human BAP31 and respiratory syncytial virus small hydrophobic (SH) protein.  |  Li, Y., et al. 2015. Virology. 482: 105-10. PMID: 25854864
3. BAP31, a promising target for the immunotherapy of malignant melanomas.  |  Yu, S., et al. 2015. J Exp Clin Cancer Res. 34: 36. PMID: 25903101
4. A BAP31 intrabody induces gastric cancer cell death by inhibiting p27kip1 proteasome degradation.  |  Chen, J., et al. 2019. Int J Cancer. 144: 2051-2062. PMID: 30338855
5. BAP31 regulates mitochondrial function via interaction with Tom40 within ER-mitochondria contact sites.  |  Namba, T. 2019. Sci Adv. 5: eaaw1386. PMID: 31206022
6. Knockdown of BAP31 Overcomes Hepatocellular Carcinoma Doxorubicin Resistance through Downregulation of Survivin.  |  Liu, J., et al. 2023. Int J Mol Sci. 24: PMID: 37108785
7. HNF4A-BAP31-VDAC1 axis synchronously regulates cell proliferation and ferroptosis in gastric cancer.  |  Zhou, Q., et al. 2023. Cell Death Dis. 14: 356. PMID: 37296105
8. BAP31 Promotes Angiogenesis via Galectin-3 Upregulation in Neuroblastoma.  |  Namusamba, M., et al. 2024. Int J Mol Sci. 25: PMID: 38474195
9. The specificity of association of the IgD molecule with the accessory proteins BAP31/BAP29 lies in the IgD transmembrane sequence.  |  Adachi, T., et al. 1996. EMBO J. 15: 1534-41. PMID: 8612576
10. p28 Bap31, a Bcl-2/Bcl-XL- and procaspase-8-associated protein in the endoplasmic reticulum.  |  Ng, FW., et al. 1997. J Cell Biol. 139: 327-38. PMID: 9334338
11. Export of cellubrevin from the endoplasmic reticulum is controlled by BAP31.  |  Annaert, WG., et al. 1997. J Cell Biol. 139: 1397-410. PMID: 9396746
12. Rapid cytochrome c release, activation of caspases 3, 6, 7 and 8 followed by Bap31 cleavage in HeLa cells treated with photodynamic therapy.  |  Granville, DJ., et al. 1998. FEBS Lett. 437: 5-10. PMID: 9804161

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-BAP31 Antibody (D-6) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-BAP31 Antibody (D-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

BAP31 Antibody (D-6) Specifications

Alternate Product Name: BAP31

Product Clone Name: D-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 157-246

Epitope Target: BAP31

Epitope Species: human

Product Reactivity/Detection: BAP31

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for BAP31 Antibody (D-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin