Bak Antibody (C-3): sc-518110
- Bak Antibody (C-3) is a mouse monoclonal IgG1 κ Bak antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 72-94 within an internal region of Bak of human origin
- recommended for detection of Bak of human origin by WB, IP, IF and ELISA
- Anti-Bak Antibody (C-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Bak Antibody (C-3) for WB applications. These reagents are now offered in bundles with Bak Antibody (C-3) (see ordering information below).
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Bak Antibody (C-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects Bak of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Bak (C-3) antibody is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Bak, a member of the Bcl-2 family of proteins, plays a crucial role in regulating apoptosis, which maintains cellular homeostasis and tissue integrity. Bak (C-3) monoclonal antibody targets the protein in the mitochondrial membrane, where Bak releases cytochrome c and other pro-apoptotic factors into the cytosol, promoting apoptotic cell death in response to cellular stress or damage. This mitochondrial localization positions Bak as a key player in the intrinsic pathway of apoptosis, effectively counteracting anti-apoptotic signals from other Bcl-2 family members, such as Bcl-2 itself. Widespread expression of Bak across various cell types highlights its importance in apoptosis regulation, making Bak a critical target for studies related to cancer, neurodegenerative diseases, and other conditions where apoptosis is dysregulated.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Bak Antibody (C-3) References:
- Pharmacologic Reduction of Mitochondrial Iron Triggers a Noncanonical BAX/BAK-Dependent Cell Death. | Garciaz, S., et al. 2022. Cancer Discov. 12: 774-791. PMID: 34862195
- Pro-apoptotic complexes of BAX and BAK on the outer mitochondrial membrane. | Wolf, P., et al. 2022. Biochim Biophys Acta Mol Cell Res. 1869: 119317. PMID: 35752202
- A BAK subdomain that binds mitochondrial lipids selectively and releases cytochrome C. | Dai, H., et al. 2023. Cell Death Differ. 30: 794-808. PMID: 36376382
- Mitophagy restricts BAX/BAK-independent, Parkin-mediated apoptosis. | Quarato, G., et al. 2023. Sci Adv. 9: eadg8156. PMID: 37224250
- BAK contributes critically to necrosis and infarct generation during reperfused myocardial infarction. | Qin, D., et al. 2023. J Mol Cell Cardiol. 184: 1-12. PMID: 37709008
- Investigation of BAK, BAX and MAD2L1 gene expression in human aneuploid blastocysts. | Ahmed, M., et al. 2023. Zygote. 31: 605-611. PMID: 37994469
- Parkin-mediated ubiquitination inhibits BAK apoptotic activity by blocking its canonical hydrophobic groove. | Cheng, P., et al. 2023. Commun Biol. 6: 1260. PMID: 38087033
- Endogenous BAX and BAK form mosaic rings of variable size and composition on apoptotic mitochondria. | Schweighofer, SV., et al. 2024. Cell Death Differ. 31: 469-478. PMID: 38503846
- Lipid unsaturation promotes BAX and BAK pore activity during apoptosis. | Dadsena, S., et al. 2024. Nat Commun. 15: 4700. PMID: 38830851
- Inhibition of BAK-mediated apoptosis by the BH3-only protein BNIP5. | Rühl, S., et al. 2024. Cell Death Differ.. PMID: 39406920
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Bak Antibody (C-3) | sc-518110 | 200 µg/ml | $322.00 | |||
Bak Antibody (C-3): m-IgGκ BP-HRP Bundle | sc-525376 | 200 µg Ab, 40 µg BP | $361.00 | |||
Bak Antibody (C-3): m-IgG1 BP-HRP Bundle | sc-551908 | 200 µg Ab; 20 µg BP | $361.00 | |||
Bak Antibody (C-3) AC | sc-518110 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Bak Antibody (C-3) HRP | sc-518110 HRP | 200 µg/ml | $322.00 | |||
Bak Antibody (C-3) FITC | sc-518110 FITC | 200 µg/ml | $336.00 | |||
Bak Antibody (C-3) PE | sc-518110 PE | 200 µg/ml | $349.00 | |||
Bak Antibody (C-3) Alexa Fluor® 488 | sc-518110 AF488 | 200 µg/ml | $364.00 | |||
Bak Antibody (C-3) Alexa Fluor® 546 | sc-518110 AF546 | 200 µg/ml | $364.00 | |||
Bak Antibody (C-3) Alexa Fluor® 594 | sc-518110 AF594 | 200 µg/ml | $364.00 | |||
Bak Antibody (C-3) Alexa Fluor® 647 | sc-518110 AF647 | 200 µg/ml | $364.00 | |||
Bak Antibody (C-3) Alexa Fluor® 680 | sc-518110 AF680 | 200 µg/ml | $364.00 | |||
Bak Antibody (C-3) Alexa Fluor® 790 | sc-518110 AF790 | 200 µg/ml | $364.00 |