Bak Antibody (AT38E2): sc-517390

Bak Antibody (AT38E2) is a mouse monoclonal IgG2a kappa light chain antibody that detects Bak protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). Anti-Bak antibody (AT38E2) is available as non-conjugated format. Bak (AT38E2) antibody recognizes a member of the Bcl-2 family that plays a crucial role in regulating apoptosis, a process of programmed cell death essential for maintaining cellular homeostasis and tissue development. Bak (AT38E2) monoclonal antibody targets a protein that promotes apoptotic cell death in response to various pro-apoptotic signals, counteracting the protective effects of anti-apoptotic proteins like Bcl-2. Bak expression is widespread across numerous cell types, including long-lived and terminally differentiated cells, indicating vital apoptotic function across different tissues. Bak′s role in facilitating apoptosis is particularly important in cancer contexts, where cell death evasion is a hallmark of tumorigenesis. Understanding Bak dynamics and interactions with other Bcl-2 family proteins can provide insights into therapeutic strategies aimed at reactivating apoptotic pathways in cancer cells.

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Bak Antibody (AT38E2) References:

1. Role of Bcl-2 family members in anoxia induced cell death.  |  Shroff, EH., et al. 2007. Cell Cycle. 6: 807-9. PMID: 17377500
2. Structure of M11L: A myxoma virus structural homolog of the apoptosis inhibitor, Bcl-2.  |  Douglas, AE., et al. 2007. Protein Sci. 16: 695-703. PMID: 17384234
3. BCL2 protein is topographically restricted in tissues characterized by apoptotic cell death.  |  Hockenbery, DM., et al. 1991. Proc Natl Acad Sci U S A. 88: 6961-5. PMID: 1871110
4. Bax, Bak and beyond - mitochondrial performance in apoptosis.  |  Peña-Blanco, A. and García-Sáez, AJ. 2018. FEBS J. 285: 416-431. PMID: 28755482
5. BAK/BAX macropores facilitate mitochondrial herniation and mtDNA efflux during apoptosis.  |  McArthur, K., et al. 2018. Science. 359: PMID: 29472455
6. The interplay between BAX and BAK tunes apoptotic pore growth to control mitochondrial-DNA-mediated inflammation.  |  Cosentino, K., et al. 2022. Mol Cell. 82: 933-949.e9. PMID: 35120587
7. Cloning the chromosomal breakpoint of t(14;18) human lymphomas: clustering around JH on chromosome 14 and near a transcriptional unit on 18.  |  Bakhshi, A., et al. 1985. Cell. 41: 899-906. PMID: 3924412
8. The t(14;18) chromosome translocations involved in B-cell neoplasms result from mistakes in VDJ joining.  |  Tsujimoto, Y., et al. 1985. Science. 229: 1390-3. PMID: 3929382
9. Induction of apoptosis by the Bcl-2 homologue Bak.  |  Chittenden, T., et al. 1995. Nature. 374: 733-6. PMID: 7715730
10. Modulation of apoptosis by the widely distributed Bcl-2 homologue Bak.  |  Kiefer, MC., et al. 1995. Nature. 374: 736-9. PMID: 7715731
11. BH1 and BH2 domains of Bcl-2 are required for inhibition of apoptosis and heterodimerization with Bax.  |  Yin, XM., et al. 1994. Nature. 369: 321-3. PMID: 8183370
12. Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death.  |  Oltvai, ZN., et al. 1993. Cell. 74: 609-19. PMID: 8358790

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Bak Antibody (AT38E2) has 20 citations in a variety of scientific publications.

Primary antibodies like Anti-Bak Antibody (AT38E2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Bak Antibody (AT38E2) Specifications

Alternate Product Name: Bak

Product Clone Name: AT38E2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 29-187

Epitope Target: Bak

Epitope Species: human

Product Reactivity/Detection: Bak

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, FCM, ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide, 1% glycerol, and 0.1% gelatin

Research Citations: 20 citations in various scientific publications

Available Conjugates/Forms for Bak Antibody (AT38E2): N/A

The preferred Bak antibody is Bak Antibody (C-3): sc-518110.