Axin Antibody (G-3): sc-518090

Axin Antibody (G-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects Axin in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Axin antibody (G-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Axin plays a crucial role in the regulation of the Wnt signaling pathway, which is essential for various cellular processes, including cell proliferation, differentiation, and migration. Axin functions as a scaffold that facilitates the formation of a complex with β-catenin, adenomatous polyposis coli (APC), and glycogen synthase kinase 3 beta (GSK-3β), promoting the degradation of β-catenin. This degradation is vital for preventing aberrant Wnt signaling, which can lead to developmental disorders and cancer. Additionally, Axin′s structural similarity to Conductin, which shares 45% identity, suggests that Axin may have overlapping functions in Wnt signaling regulation. Axin′s ability to modulate β-catenin levels underscores its importance in maintaining cellular homeostasis and its potential as a therapeutic target in Wnt-related diseases.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Axin Antibody (G-3) References:

1. Modulation of Wnt signaling by Axin and Axil.  |  Kikuchi, A. 1999. Cytokine Growth Factor Rev. 10: 255-65. PMID: 10647780
2. Axin gene methylation status correlates with radiosensitivity of lung cancer cells.  |  Yang, LH., et al. 2013. BMC Cancer. 13: 368. PMID: 23915259
3. Axin cancer mutants form nanoaggregates to rewire the Wnt signaling network.  |  Anvarian, Z., et al. 2016. Nat Struct Mol Biol. 23: 324-32. PMID: 26974125
4. Determining AMPK Activation via the Lysosomal v-ATPase-Ragulator-AXIN/LKB1 Axis.  |  Zhang, CS., et al. 2018. Methods Mol Biol. 1732: 393-411. PMID: 29480489
5. Limited dishevelled/Axin oligomerization determines efficiency of Wnt/β-catenin signal transduction.  |  Kan, W., et al. 2020. Elife. 9: PMID: 32297861
6. Reconstitution of the destruction complex defines roles of AXIN polymers and APC in β-catenin capture, phosphorylation, and ubiquitylation.  |  Ranes, M., et al. 2021. Mol Cell. 81: 3246-3261.e11. PMID: 34352208
7. S-SCAM inhibits Axin-dependent synaptic function of GSK3β in a sex-dependent manner.  |  Kearney, G., et al. 2022. Sci Rep. 12: 4090. PMID: 35260764
8. Immunohistochemical expression of Axin-2, as an implication of the role of stem cell in scar pathogenesis and prognosis.  |  Bazid, HAS., et al. 2022. J Cosmet Dermatol. 21: 6010-6020. PMID: 35546288
9. Isoxazole 9 (ISX9), a small molecule targeting Axin, activates Wnt/β-catenin signalling and promotes hair regrowth.  |  Sayed, S., et al. 2023. Br J Pharmacol. 180: 1748-1765. PMID: 36721985
10. SUMOylation of RNF146 results in Axin degradation and activation of Wnt/β-catenin signaling to promote the progression of hepatocellular carcinoma.  |  Li, W., et al. 2023. Oncogene. 42: 1728-1740. PMID: 37029301

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Axin Antibody (G-3) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-Axin Antibody (G-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Axin Antibody (G-3) Specifications

Alternate Product Name: AXIN1

Product Clone Name: G-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 336-360

Epitope Target: Axin

Epitope Species: mouse

Product Reactivity/Detection: Axin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Axin Antibody (G-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin