AUH Antibody (B-8): sc-518216
- AUH Antibody (B-8) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 321-339 of AUH of human origin
- recommended for detection of AUH of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-AUH Antibody (B-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for AUH Antibody (B-8) for WB applications. These reagents are now offered in bundles with AUH Antibody (B-8) (see ordering information below).
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SEE ALSO...
AUH Antibody (B-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects AUH in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). AUH antibody (B-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. AUH, also known as AU-binding protein or mitochondrial methylglutaconyl-CoA hydratase, consists of 339 amino acids and belongs to the enoyl-CoA hydratase/isomerase family. AUH plays a crucial role in the amino acid degradation pathway by catalyzing the conversion of 3-methylglutaconyl-CoA to 3-hydroxy-3-methylglutaryl-CoA and water, a process vital for energy metabolism and proper breakdown of certain amino acids. AUH is localized in mitochondria, where AUH has shown very low enoyl-CoA hydratase activity, and exists as two isoforms due to alternative splicing, forming a homohexamer structure. Deficiencies in AUH can lead to 3-methylglutaconic aciduria type 1 (MGA1), a metabolic disorder characterized by severe symptoms, including coma, psychomotor retardation, and metabolic acidosis, highlighting AUH′s importance in maintaining metabolic health.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
AUH Antibody (B-8) References:
- Characterisation and mitochondrial localisation of AUH, an AU-specific RNA-binding enoyl-CoA hydratase. | Brennan, LE., et al. 1999. Gene. 228: 85-91. PMID: 10072761
- Crystal structure of human AUH protein, a single-stranded RNA binding homolog of enoyl-CoA hydratase. | Kurimoto, K., et al. 2001. Structure. 9: 1253-63. PMID: 11738050
- 3-Methylglutaconic aciduria type I is caused by mutations in AUH. | IJlst, L., et al. 2002. Am J Hum Genet. 71: 1463-6. PMID: 12434311
- Mutations in the AUH gene cause 3-methylglutaconic aciduria type I. | Ly, TB., et al. 2003. Hum Mutat. 21: 401-7. PMID: 12655555
- 3-methylglutaconic aciduria type I in a boy with fever-associated seizures. | Illsinger, S., et al. 2004. Pediatr Neurol. 30: 213-5. PMID: 15033206
- Biochemical characterization of human 3-methylglutaconyl-CoA hydratase and its role in leucine metabolism. | Mack, M., et al. 2006. FEBS J. 273: 2012-22. PMID: 16640564
- AU-rich RNA-binding induces changes in the quaternary structure of AUH. | Kurimoto, K., et al. 2009. Proteins. 75: 360-72. PMID: 18831052
- AUH, a gene encoding an AU-specific RNA binding protein with intrinsic enoyl-CoA hydratase activity. | Nakagawa, J., et al. 1995. Proc Natl Acad Sci U S A. 92: 2051-5. PMID: 7892223
- A 20-amino-acid autonomous RNA-binding domain contained in an enoyl-CoA hydratase. | Nakagawa, J. and Moroni, C. 1997. Eur J Biochem. 244: 890-9. PMID: 9108262
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AUH Antibody (B-8) | sc-518216 | 200 µg/ml | $322.00 | |||
AUH Antibody (B-8): m-IgG Fc BP-HRP Bundle | sc-551270 | 200 µg Ab; 10 µg BP | $361.00 | |||
AUH Antibody (B-8): m-IgG1 BP-HRP Bundle | sc-551268 | 200 µg Ab; 20 µg BP | $361.00 | |||
AUH Antibody (B-8): m-IgGκ BP-HRP Bundle | sc-551269 | 200 µg Ab; 40 µg BP | $361.00 | |||
AUH Antibody (B-8) AC | sc-518216 AC | 500 µg/ml, 25% agarose | $424.00 | |||
AUH Antibody (B-8) HRP | sc-518216 HRP | 200 µg/ml | $322.00 | |||
AUH Antibody (B-8) FITC | sc-518216 FITC | 200 µg/ml | $336.00 | |||
AUH Antibody (B-8) PE | sc-518216 PE | 200 µg/ml | $349.00 | |||
AUH Antibody (B-8) Alexa Fluor® 488 | sc-518216 AF488 | 200 µg/ml | $364.00 | |||
AUH Antibody (B-8) Alexa Fluor® 546 | sc-518216 AF546 | 200 µg/ml | $364.00 | |||
AUH Antibody (B-8) Alexa Fluor® 594 | sc-518216 AF594 | 200 µg/ml | $364.00 | |||
AUH Antibody (B-8) Alexa Fluor® 647 | sc-518216 AF647 | 200 µg/ml | $364.00 | |||
AUH Antibody (B-8) Alexa Fluor® 680 | sc-518216 AF680 | 200 µg/ml | $364.00 | |||
AUH Antibody (B-8) Alexa Fluor® 790 | sc-518216 AF790 | 200 µg/ml | $364.00 |