ATPAF1 Antibody (E-12): sc-398684
- ATPAF1 Antibody (E-12) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- raised against amino acids 74-230 mapping within an internal region of ATPAF1 of human origin
- recommended for detection of ATPAF1 of human origin by WB, IP, IF and ELISA
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ATPAF1 Antibody (E-12) for WB applications. These reagents are now offered in bundles with ATPAF1 Antibody (E-12) (see ordering information below).
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ATPAF1 Antibody (E-12) is a mouse monoclonal IgG1 antibody that detects ATPAF1 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ATPAF1, also known as ATP synthase mitochondrial F1 complex assembly factor 1, plays a crucial role in the assembly of the mitochondrial ATP synthase complex, specifically facilitating the incorporation of F1-beta and F1-alpha subunits into the ATPase structure. This 328 amino acid protein is essential for the proper functioning of ATP synthase, which synthesizes ATP by transducing energy from electrochemical proton gradients across the mitochondrial membrane. ATPAF1 is highly conserved across eukaryotic species, indicating a fundamental role in cellular energy metabolism. Disruptions in ATPAF1 function can lead to ATP synthase deficiencies, which may manifest in various clinical conditions where the biochemical basis remains elusive. Anti-ATPAF1 antibody (E-12) serves as a valuable tool for researchers investigating mitochondrial function and related disorders, providing insights into the assembly and regulation of this vital enzyme complex.
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ATPAF1 Antibody (E-12) References:
- Atp11p and Atp12p are assembly factors for the F(1)-ATPase in human mitochondria. | Wang, ZG., et al. 2001. J Biol Chem. 276: 30773-8. PMID: 11410595
- Differential expression of ATPAF1 and ATPAF2 genes encoding F(1)-ATPase assembly proteins in mouse tissues. | Picková, A., et al. 2003. FEBS Lett. 551: 42-6. PMID: 12965202
- OGFOD1, a member of the 2-oxoglutarate and iron dependent dioxygenase family, functions in ischemic signaling. | Saito, K., et al. 2010. FEBS Lett. 584: 3340-7. PMID: 20579638
- X-linked cholestasis in mouse due to mutations of the P4-ATPase ATP11C. | Siggs, OM., et al. 2011. Proc Natl Acad Sci U S A. 108: 7890-5. PMID: 21518881
- Identification of ATPAF1 as a novel candidate gene for asthma in children. | Schauberger, EM., et al. 2011. J Allergy Clin Immunol. 128: 753-760.e11. PMID: 21696813
- A novel approach to identify driver genes involved in androgen-independent prostate cancer. | Schinke, EN., et al. 2014. Mol Cancer. 13: 120. PMID: 24885513
- Protein coingestion with alcohol following strenuous exercise attenuates alcohol-induced intramyocellular apoptosis and inhibition of autophagy. | Smiles, WJ., et al. 2016. Am J Physiol Endocrinol Metab. 311: E836-E849. PMID: 27677502
- Systematic Analysis of the Expression of the Mitochondrial ATP Synthase (Complex V) Subunits in Clear Cell Renal Cell Carcinoma. | Brüggemann, M., et al. 2017. Transl Oncol. 10: 661-668. PMID: 28672194
- ATPAF1 deficiency impairs ATP synthase assembly and mitochondrial respiration. | Zhou, Z., et al. 2021. Mitochondrion. 60: 129-141. PMID: 34375736
- LINC00115 promotes gastric cancer partly by the miR-212-5p/ATPAF1 axis. | Zhu, Q., et al. 2023. An Acad Bras Cienc. 95: e20230480. PMID: 38088732
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ATPAF1 Antibody (E-12) | sc-398684 | 200 µg/ml | $322.00 | |||
ATPAF1 Antibody (E-12): m-IgG Fc BP-HRP Bundle | sc-538501 | 200 µg Ab; 10 µg BP | $361.00 | |||
ATPAF1 Antibody (E-12): m-IgGκ BP-HRP Bundle | sc-536127 | 200 µg Ab; 40 µg BP | $361.00 | |||
ATPAF1 Antibody (E-12): m-IgG1 BP-HRP Bundle | sc-545734 | 200 µg Ab; 20 µg BP | $361.00 |