ASPP1 Antibody (54.2): sc-53860

ASPP1 Antibody (54.2) is a mouse monoclonal IgG1 antibody that detects ASPP1 in mouse, rat, and human samples through applications such as western blotting (WB) and immunoprecipitation (IP). ASPP1 plays a crucial role in regulating apoptosis by interacting with the tumor suppressor protein p53, enhancing its ability to induce cell death in response to apoptotic signals while not affecting cell cycle arrest. This function is particularly important in the context of cancer, as ASPP1 amplifies the DNA binding and transactivation capabilities of p53 on the promoters of pro-apoptotic genes, thereby promoting tumor suppression. Notably, ASPP1 is predominantly located in the cytoplasm, with a smaller fraction found in the nucleus, which is significant for its interaction with p53 and its role in apoptosis. ASPP1 expression is often downregulated in human breast carcinomas that express wild-type p53, suggesting that defects in the PPP1R13B gene, which encodes ASPP1, may contribute to breast cancer development. The deduced ASPP1 protein consists of 1,090 amino acid residues, and structural integrity is vital for function in apoptosis regulation. Anti-ASPP1 antibody (54.2) is an essential tool for researchers studying the mechanisms of p53-mediated apoptosis and its implications in cancer biology.

The preferred ASPP1 antibody is ASPP1 Antibody (LX011): sc-53903.