ASPP1 Antibody (LX011): sc-53903
- ASPP1 Antibody (LX011) is a mouse monoclonal IgG1 κ ASPP1 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against a recombinant protein containing the N-terminal region of ASPP1 human origin
- ASPP1 Antibody (LX011) is recommended for detection of ASPP1 of mouse, rat and human origin by WB, IP, IF and IHC(P)
- Anti-ASPP1 Antibody (LX011) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for ASPP1 Antibody (LX011) for WB and IHC(P) applications. These reagents are now offered in bundles with ASPP1 Antibody (LX011) (see ordering information below).
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ASPP1 Antibody (LX011) is a mouse monoclonal IgG1 kappa light chain antibody that detects ASPP1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. ASPP1 Antibody (LX011) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ASPP1 plays a crucial role in regulating apoptosis by enhancing the apoptotic function of the tumor suppressor protein p53, particularly in response to cellular stress. ASPP1 (LX011) monoclonal antibody helps detect this interaction that prevents the survival of damaged cells, thereby contributing to cancer prevention. ASPP1 amplifies the DNA binding and transactivation capabilities of p53 on the promoters of pro-apoptotic genes, which is essential for effective apoptosis. ASPP1 is predominantly located in the cytoplasm, with a portion also found in the nucleus, highlighting a dynamic role in cellular signaling pathways. ASPP1 expression is often downregulated in human breast carcinomas that express wild-type p53, suggesting significant involvement in tumor suppression mechanisms. Defects in the PPP1R13B gene, which encodes ASPP1, have been implicated in breast cancer development, underscoring the importance in maintaining cellular integrity. The deduced ASPP1 protein consists of 1,090 amino acid residues, reflecting complex structure and functional capabilities.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ASPP1 Antibody (LX011) References:
- ASPP proteins specifically stimulate the apoptotic function of p53. | Samuels-Lev, Y., et al. 2001. Mol Cell. 8: 781-94. PMID: 11684014
- ASPP1 and ASPP2: common activators of p53 family members. | Bergamaschi, D., et al. 2004. Mol Cell Biol. 24: 1341-50. PMID: 14729977
- ASPP1 and ASPP2 are new transcriptional targets of E2F. | Fogal, V., et al. 2005. Cell Death Differ. 12: 369-76. PMID: 15731768
- Mdm2 and mdmX prevent ASPP1 and ASPP2 from stimulating p53 without targeting p53 for degradation. | Bergamaschi, D., et al. 2005. Oncogene. 24: 3836-41. PMID: 15782125
- ASPP1, a common activator of TP53, is inactivated by aberrant methylation of its promoter in acute lymphoblastic leukemia. | Agirre, X., et al. 2006. Oncogene. 25: 1862-70. PMID: 16314841
- The tumour-suppressor protein ASPP1 is nuclear in human germ cells and can modulate ratios of CD44 exon V5 spliced isoforms in vivo. | Thornton, JK., et al. 2006. Oncogene. 25: 3104-12. PMID: 16474851
- Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. | Nagase, T., et al. 1998. DNA Res. 5: 277-86. PMID: 9872452
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ASPP1 Antibody (LX011) | sc-53903 | 200 µg/ml | $322.00 | |||
ASPP1 Antibody (LX011): m-IgG Fc BP-HRP Bundle | sc-526849 | 200 µg Ab; 10 µg BP | $361.00 | |||
ASPP1 Antibody (LX011): m-IgG1 BP-HRP Bundle | sc-532222 | 200 µg Ab; 20 µg BP | $361.00 | |||
ASPP1 Antibody (LX011) AC | sc-53903 AC | 500 µg/ml, 25% agarose | $424.00 | |||
ASPP1 Antibody (LX011) HRP | sc-53903 HRP | 200 µg/ml | $322.00 | |||
ASPP1 Antibody (LX011) FITC | sc-53903 FITC | 200 µg/ml | $336.00 | |||
ASPP1 Antibody (LX011) PE | sc-53903 PE | 200 µg/ml | $349.00 | |||
ASPP1 Antibody (LX011) Alexa Fluor® 488 | sc-53903 AF488 | 200 µg/ml | $364.00 | |||
ASPP1 Antibody (LX011) Alexa Fluor® 546 | sc-53903 AF546 | 200 µg/ml | $364.00 | |||
ASPP1 Antibody (LX011) Alexa Fluor® 594 | sc-53903 AF594 | 200 µg/ml | $364.00 | |||
ASPP1 Antibody (LX011) Alexa Fluor® 647 | sc-53903 AF647 | 200 µg/ml | $364.00 | |||
ASPP1 Antibody (LX011) Alexa Fluor® 680 | sc-53903 AF680 | 200 µg/ml | $364.00 | |||
ASPP1 Antibody (LX011) Alexa Fluor® 790 | sc-53903 AF790 | 200 µg/ml | $364.00 |