ARFGAP1 Antibody (C-4): sc-271303
- ARFGAP1 Antibody (C-4) is a mouse monoclonal IgG1 κ ARFGAP1 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 1-286 mapping at the N-terminus of ARFGAP1 of human origin
- ARFGAP1 Antibody (C-4) is recommended for detection of ARFGAP1 of human origin by WB, IP, IF and ELISA
- Anti-ARFGAP1 Antibody (C-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for ARFGAP1 Antibody (C-4) for WB applications. This reagent is now offered in a bundle with ARFGAP1 Antibody (C-4) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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ARFGAP1 Antibody (C-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects ARFGAP1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-ARFGAP1 antibody (C-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ARFGAP1, also known as ADP-ribosylation factor GTPase activating protein 1, consists of 406 amino acids and features a single ARF-GAP domain, primarily localized in the cytoplasm and the Golgi apparatus. ARFGAP1 functions as a GTPase-activating protein (GAP) by interacting with ARF1 to promote GTP hydrolysis bound to ARF1, a process essential for coat protein dissociation from Golgi structures and subsequent fusion of Golgi vesicles with target compartments. ARFGAP1′s activity can be modulated by various lipids; phosphatidylcholine inhibits while phosphoinositides stimulate ARFGAP1, which can lead to Golgi apparatus redistribution to the endoplasmic reticulum when overexpressed. Multiple isoforms of ARFGAP1 arise from alternative splicing, contributing to functional diversity and regulatory mechanisms within cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ARFGAP1 Antibody (C-4) References:
- Characterization, chromosomal assignment, and tissue expression of a novel human gene belonging to the ARF GAP family. | Zhang, C., et al. 2000. Genomics. 63: 400-8. PMID: 10704287
- KDEL-cargo regulates interactions between proteins involved in COPI vesicle traffic: measurements in living cells using FRET. | Majoul, I., et al. 2001. Dev Cell. 1: 139-53. PMID: 11703931
- ARFGAP1 promotes the formation of COPI vesicles, suggesting function as a component of the coat. | Yang, JS., et al. 2002. J Cell Biol. 159: 69-78. PMID: 12379802
- GAP control: regulating the regulators of small GTPases. | Bernards, A. and Settleman, J. 2004. Trends Cell Biol. 14: 377-85. PMID: 15246431
- Golgi localization determinants in ArfGAP1 and in new tissue-specific ArfGAP1 isoforms. | Parnis, A., et al. 2006. J Biol Chem. 281: 3785-92. PMID: 16316994
- SMAP2, a novel ARF GTPase-activating protein, interacts with clathrin and clathrin assembly protein and functions on the AP-1-positive early endosome/trans-Golgi network. | Natsume, W., et al. 2006. Mol Biol Cell. 17: 2592-603. PMID: 16571680
- Insights into COPI coat assembly and function in living cells. | Lippincott-Schwartz, J. and Liu, W. 2006. Trends Cell Biol. 16: e1-4. PMID: 16956762
- Kinetic analysis of Arf GAP1 indicates a regulatory role for coatomer. | Luo, R. and Randazzo, PA. 2008. J Biol Chem. 283: 21965-77. PMID: 18541532
- The ARF1 GTPase-activating protein: zinc finger motif and Golgi complex localization. | Cukierman, E., et al. 1995. Science. 270: 1999-2002. PMID: 8533093
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ARFGAP1 Antibody (C-4) | sc-271303 | 200 µg/ml | $322.00 | |||
ARFGAP1 Antibody (C-4): m-IgGκ BP-HRP Bundle | sc-521906 | 200 µg Ab, 40 µg BP | $361.00 | |||
ARFGAP1 Antibody (C-4) AC | sc-271303 AC | 500 µg/ml, 25% agarose | $424.00 | |||
ARFGAP1 Antibody (C-4) HRP | sc-271303 HRP | 200 µg/ml | $322.00 | |||
ARFGAP1 Antibody (C-4) FITC | sc-271303 FITC | 200 µg/ml | $336.00 | |||
ARFGAP1 Antibody (C-4) PE | sc-271303 PE | 200 µg/ml | $349.00 | |||
ARFGAP1 Antibody (C-4) Alexa Fluor® 488 | sc-271303 AF488 | 200 µg/ml | $364.00 | |||
ARFGAP1 Antibody (C-4) Alexa Fluor® 546 | sc-271303 AF546 | 200 µg/ml | $364.00 | |||
ARFGAP1 Antibody (C-4) Alexa Fluor® 594 | sc-271303 AF594 | 200 µg/ml | $364.00 | |||
ARFGAP1 Antibody (C-4) Alexa Fluor® 647 | sc-271303 AF647 | 200 µg/ml | $364.00 | |||
ARFGAP1 Antibody (C-4) Alexa Fluor® 680 | sc-271303 AF680 | 200 µg/ml | $364.00 | |||
ARFGAP1 Antibody (C-4) Alexa Fluor® 790 | sc-271303 AF790 | 200 µg/ml | $364.00 |