ARF6 Antibody (3A-1): sc-7971

ARF6 Antibody (3A-1) is a mouse monoclonal IgG2b kappa light chain antibody that detects ARF6 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). ARF6 (3A-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ARF6, a member of the ADP-ribosylation factor family, plays a crucial role in regulating membrane trafficking and cytoskeletal dynamics, which are essential for maintaining cellular homeostasis and facilitating intracellular communication. ARF6 is localized primarily at the plasma membrane and endosomal compartments, where ARF6 orchestrates the recycling of membrane proteins and lipids, thereby influencing processes such as endocytosis and exocytosis. This localization is vital for the proper functioning of various cellular processes, including cell migration, adhesion, and the formation of membrane protrusions. The human ARF6 gene consists of five exons and four introns, encoding a 175 amino acid protein that is integral to the coordination of vesicle transport and the maintenance of organelle integrity. Anti-ARF6 antibody (3A-1) enables valuable insights into the mechanisms of cellular signaling and trafficking, which are critical for understanding various physiological and pathological conditions.

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ARF6 Antibody (3A-1) References:

1. Activated ADP-ribosylation factor assembles distinct pools of actin on golgi membranes.  |  Fucini, RV., et al. 2000. J Biol Chem. 275: 18824-9. PMID: 10777475
2. The gamma-subunit of the coatomer complex binds Cdc42 to mediate transformation.  |  Wu, WJ., et al. 2000. Nature. 405: 800-4. PMID: 10866202
3. Golgi vesicle proteins are linked to the assembly of an actin complex defined by mAbp1.  |  Fucini, RV., et al. 2002. Mol Biol Cell. 13: 621-31. PMID: 11854417
4. ARF6 and Rab11 as intrinsic regulators of axon regeneration.  |  Nieuwenhuis, B. and Eva, R. 2020. Small GTPases. 11: 392-401. PMID: 29772958
5. Arf6 regulates energy metabolism in neutrophils.  |  Gamara, J., et al. 2021. Free Radic Biol Med. 172: 550-561. PMID: 34245858
6. Targeting EGFR-dependent tumors by disrupting an ARF6-mediated sorting system.  |  Guo, H., et al. 2022. Nat Commun. 13: 6004. PMID: 36224181
7. Arf6 as a therapeutic target: Structure, mechanism, and inhibitors.  |  Sun, D., et al. 2023. Acta Pharm Sin B. 13: 4089-4104. PMID: 37799386
8. Platelet-derived extracellular vesicles aggravate septic acute kidney injury via delivering ARF6.  |  Lu, X., et al. 2023. Int J Biol Sci. 19: 5055-5073. PMID: 37928258
9. The amino terminus of ADP-ribosylation factor (ARF) 1 is essential for interaction with Gs and ARF GTPase-activating protein.  |  Randazzo, PA., et al. 1994. J Biol Chem. 269: 29490-4. PMID: 7961931
10. Structure of the human ADP-ribosylation factor 1 complexed with GDP.  |  Amor, JC., et al. 1994. Nature. 372: 704-8. PMID: 7990966
11. Mammalian Cdc42 is a brefeldin A-sensitive component of the Golgi apparatus.  |  Erickson, JW., et al. 1996. J Biol Chem. 271: 26850-4. PMID: 8900167
12. ADP ribosylation factor regulates spectrin binding to the Golgi complex.  |  Godi, A., et al. 1998. Proc Natl Acad Sci U S A. 95: 8607-12. PMID: 9671725

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ARF6 Antibody (3A-1) has 108 citations in a variety of scientific publications.

Primary antibodies like Anti-ARF6 Antibody (3A-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ARF6 Antibody (3A-1) Specifications

Alternate Product Name: ARF6

Product Clone Name: 3A-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-174

Epitope Target: ARF6

Epitope Species: human

Product Reactivity/Detection: ARF6

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 108 citations in various scientific publications

Available Conjugates/Forms for ARF6 Antibody (3A-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin