ARA54 Antibody (A-3): sc-166236

ARA54 Antibody (A-3) is a mouse monoclonal IgG1 antibody that detects ARA54 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ARA54 plays a crucial role as a coactivator of the androgen receptor (AR), which is essential for mediating the effects of androgens like testosterone and dihydrotestosterone on male reproductive physiology and phenotype. ARA54 is predominantly expressed in tissues such as the testis, thymus, spleen, colon, prostate, and uterus, highlighting its significance in various biological processes. ARA54 contains a RING finger domain, which is important for AR interaction and involvement in chromatin remodeling through association with the SNF/SWI complex. This interaction is facilitated by the FXXLF motif found in AR coactivators, which is critical for binding to AR, although this binding does not influence transcriptional activity. Understanding ARA54 function and interactions is vital for elucidating the mechanisms of androgen signaling and its implications in male reproductive health and related disorders.

The preferred ARA54 antibody is ARA54 Antibody (B-10): sc-376701.