ARA54 Antibody (B-10): sc-376701
- ARA54 Antibody (B-10) is a mouse monoclonal IgG1 κ ARA54 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 2-27 at the N-terminus of ARA54 of human origin
- ARA54 Antibody (B-10) is recommended for detection of ARA54 of mouse, rat, human and avian origin by WB, IP, IF and ELISA; also reactive with additional species, including and canine, bovine, porcine and avian
- TransCruz reagent for ChIP application (sc-376701 X, 200 µg/0.1 ml)
- Anti-ARA54 Antibody (B-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ARA54 Antibody (B-10) for WB applications. These reagents are now offered in bundles with ARA54 Antibody (B-10) (see ordering information below).
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ARA54 Antibody (B-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects ARA54 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ARA54 (B-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ARA54 functions as a coactivator of the androgen receptor (AR), which mediates the effects of androgens like testosterone and dihydrotestosterone on male reproductive physiology and phenotype. ARA54 contains a RING finger domain that enhances AR activity, particularly in tissues such as the testis, thymus, spleen, colon, prostate, and uterus, where ARA54 is expressed at high levels. The interaction between ARA54 and AR is facilitated by the FXXLF motif found in AR coactivators, which is critical for binding to the receptor, although this motif does not influence transcriptional activity. Understanding ARA54 and its interaction with AR helps illuminate the molecular mechanisms underlying androgen signaling and its implications in male reproductive health.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ARA54 Antibody (B-10) References:
- Cloning and characterization of human prostate coactivator ARA54, a novel protein that associates with the androgen receptor. | Kang, HY., et al. 1999. J Biol Chem. 274: 8570-6. PMID: 10085091
- Isolation and characterization of a novel human gene (HFB30) which encodes a protein with a RING finger motif. | Ueki, N., et al. 1999. Biochim Biophys Acta. 1445: 232-6. PMID: 10320776
- The FXXLF motif mediates androgen receptor-specific interactions with coregulators. | He, B., et al. 2002. J Biol Chem. 277: 10226-35. PMID: 11779876
- A putative nuclear receptor coactivator (TMF/ARA160) associates with hbrm/hSNF2 alpha and BRG-1/hSNF2 beta and localizes in the Golgi apparatus. | Mori, K. and Kato, H. 2002. FEBS Lett. 520: 127-32. PMID: 12044884
- Cloning and chromosomal mapping of a human immunodeficiency virus 1 'TATA' element modulatory factor. | Garcia, JA., et al. 1992. Proc Natl Acad Sci U S A. 89: 9372-6. PMID: 1409643
- Familial incomplete male pseudohermaphroditism, type 2. Decreased dihydrotestosterone formation in pseudovaginal perineoscrotal hypospadias. | Walsh, PC., et al. 1974. N Engl J Med. 291: 944-9. PMID: 4413434
- Steroid 5alpha-reductase deficiency in man: an inherited form of male pseudohermaphroditism. | Imperato-McGinley, J., et al. 1974. Science. 186: 1213-5. PMID: 4432067
- The androgen receptor: an overview. | Zhou, ZX., et al. 1994. Recent Prog Horm Res. 49: 249-74. PMID: 8146426
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ARA54 Antibody (B-10) | sc-376701 | 200 µg/ml | $322.00 | |||
ARA54 Antibody (B-10): m-IgGκ BP-HRP Bundle | sc-523052 | 200 µg Ab, 40 µg BP | $361.00 | |||
ARA54 Antibody (B-10): m-IgG1 BP-HRP Bundle | sc-533546 | 200 µg Ab; 20 µg BP | $361.00 | |||
ARA54 Antibody (B-10) X | sc-376701 X | 200 µg/0.1 ml | $322.00 | |||
ARA54 Antibody (B-10) AC | sc-376701 AC | 500 µg/ml, 25% agarose | $424.00 | |||
ARA54 Antibody (B-10) HRP | sc-376701 HRP | 200 µg/ml | $322.00 | |||
ARA54 Antibody (B-10) FITC | sc-376701 FITC | 200 µg/ml | $336.00 | |||
ARA54 Antibody (B-10) PE | sc-376701 PE | 200 µg/ml | $349.00 | |||
ARA54 Antibody (B-10) Alexa Fluor® 488 | sc-376701 AF488 | 200 µg/ml | $364.00 | |||
ARA54 Antibody (B-10) Alexa Fluor® 546 | sc-376701 AF546 | 200 µg/ml | $364.00 | |||
ARA54 Antibody (B-10) Alexa Fluor® 594 | sc-376701 AF594 | 200 µg/ml | $364.00 | |||
ARA54 Antibody (B-10) Alexa Fluor® 647 | sc-376701 AF647 | 200 µg/ml | $364.00 | |||
ARA54 Antibody (B-10) Alexa Fluor® 680 | sc-376701 AF680 | 200 µg/ml | $364.00 | |||
ARA54 Antibody (B-10) Alexa Fluor® 790 | sc-376701 AF790 | 200 µg/ml | $364.00 | |||
ARA54 (B-10) Neutralizing Peptide | sc-376701 P | 100 µg/0.5 ml | $69.00 |