Aquaporin 2/AQP2 Antibody (E-2): sc-515770

AQP2 Antibody (E-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects AQP2 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-AQP2 antibody (E-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. AQP2 plays a crucial role in regulating water balance in the body, specifically by facilitating water reabsorption in the kidney collecting ducts in response to vasopressin. This function is vital for maintaining homeostasis, particularly in conditions of dehydration or increased plasma osmolality. AQP2 is unique among aquaporins as the only water channel directly regulated by vasopressin, which enhances activity and promotes urine concentration. Proper functioning of AQP2 is essential for preventing conditions such as diabetes insipidus, where the body fails to concentrate urine, leading to excessive urination and thirst. Understanding AQP2 regulation is important for developing therapeutic strategies for kidney-related disorders and managing fluid balance in various clinical settings.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Aquaporin 2/AQP2 Antibody (E-2) References:

1. Actin directly interacts with different membrane channel proteins and influences channel activities: AQP2 as a model.  |  Sasaki, S., et al. 2014. Biochim Biophys Acta. 1838: 514-20. PMID: 23770358
2. Regulation of AQP2 in Collecting Duct: An emphasis on the Effects of Angiotensin II or Aldosterone.  |  Lee, BH. and Kwon, TH. 2007. Electrolyte Blood Press. 5: 15-22. PMID: 24459495
3. AQP2 Abundance is Regulated by the E3-Ligase CHIP Via HSP70.  |  Centrone, M., et al. 2017. Cell Physiol Biochem. 44: 515-531. PMID: 29145196
4. Phosphorylation of human AQP2 and its role in trafficking.  |  Törnroth-Horsefield, S. 2020. Vitam Horm. 112: 95-117. PMID: 32061351
5. AQP2: Mutations Associated with Congenital Nephrogenic Diabetes Insipidus and Regulation by Post-Translational Modifications and Protein-Protein Interactions.  |  Gao, C., et al. 2020. Cells. 9: PMID: 32993088
6. Aqp2+ Progenitor Cells Maintain and Repair Distal Renal Segments.  |  Gao, C., et al. 2022. J Am Soc Nephrol. 33: 1357-1376. PMID: 35318267
7. AQP2 Promotes Astrocyte Activation by Modulating the TLR4/NFκB-p65 Pathway Following Intracerebral Hemorrhage.  |  Deng, S., et al. 2022. Front Immunol. 13: 847360. PMID: 35386692
8. Intracellular sites of AQP2 S256 phosphorylation identified using inhibitors of the AQP2 recycling itinerary.  |  Cheung, PW., et al. 2023. Am J Physiol Renal Physiol. 324: F152-F167. PMID: 36454701
9. Genome-Engineered mpkCCDc14 Cells as a New Resource for Studying AQP2.  |  Jang, HJ., et al. 2023. Int J Mol Sci. 24: PMID: 36675199
10. TNF inhibits AQP2 expression via a miR137-dependent pathway.  |  Hao, S., et al. 2024. Am J Physiol Renal Physiol. 326: F152-F164. PMID: 37969102

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Aquaporin 2/AQP2 Antibody (E-2) has 68 citations in a variety of scientific publications.

Primary antibodies like Anti-Aquaporin 2/AQP2 Antibody (E-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Aquaporin 2/AQP2 Antibody (E-2) Specifications

Alternate Product Name: aquaporin 2

Product Clone Name: E-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 232-271

Epitope Target: AQP2

Epitope Species: human

Product Reactivity/Detection: AQP2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 68 citations in various scientific publications

Available Conjugates/Forms for Aquaporin 2/AQP2 Antibody (E-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Aquaporin 2/AQP2 antibody is Aquaporin 2/AQP2 Antibody (E13-21): sc-47710.