Anti-APEH Antibody (B-2) recommended for detection of APEH of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-APEH Antibody (B-2): sc-393452

Anti-APEH Antibody (B-2) is rated 5.0 out of 5 by 2.
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Datasheets

    • APEH Antibody (B-2) is a mouse monoclonal mouse IgG2a κ (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 16-101 mapping near the N-terminus of APEH of human origin
    • recommended for detection of APEH of mouse, rat and human origin by WB, IP, IF and ELISA
    • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for APEH Antibody (B-2) for WB applications. These reagents are now offered in bundles with APEH Antibody (B-2) (see ordering information below).
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What is the recommended fixation method for immunofluorescence staining with APEH (B-2): sc-393452 monoclonal antibody?

Asked by: AbPolly
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Produced exceptional immunofluorescence cytoplasmic Produced exceptional immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-04-08
Rated 5 out of 5 by from Produced positive Western Blot data of APEH Produced positive Western Blot data of APEH expression in Jurkat and HeLa whole cell lysates and human heart and human liver tissue extracts. -SCBT QC
Date published: 2013-02-24
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