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Amylase Antibody (A-1): sc-514229

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Datasheets
  • Amylase Antibody (A-1) is a mouse monoclonal IgG1 κ, cited in 2 publications, provided at 200 µg/ml
  • specific for an epitope mapping between amino acids 483-508 at the C-terminus of Amylase of human origin
  • recommended for detection of Amylase 1, Amylase 2A and Amylase 2B of human origin, Amylase 1 and Amylase 2a5 of mouse origin, and Amylase 2 of rat origin by WB, IP, IF and ELISA
  • See Amylase (G-10): sc-46657 for Amylase antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Amylase Antibody (A-1) for WB applications. These reagents are now offered in bundles with Amylase Antibody (A-1) (see ordering information below).

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Amylase Antibody (A-1) is a mouse monoclonal IgG1 kappa light chain antibody designed to detect various forms of amylase proteins, including Amylase 1, Amylase 2A, and Amylase 2B in humans, Amylase 1 and Amylase 2a5 in mice, and Amylase 2 in rats through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA) assays. Anti-amylase antibody (A-1) targets an epitope spanning amino acids 483-508 at the C-terminus of human amylase, also known as alpha-amylase, ensuring precise recognition of the protein′s structural domains essential for enzymatic activity. Amylase proteins are characterized by complex tertiary structures, which include a highly conserved catalytic domain responsible for the hydrolysis of starch into sugars, a function critical for efficient carbohydrate metabolism and energy production. The structural integrity of amylase is vital for interaction with substrates and inhibitors, influencing activity and regulation within the digestive system and other tissues. Understanding the structural aspects of amylase is crucial for researching metabolic disorders, pancreatic function, and certain tumor pathologies, making amylase monoclonal antibody (A-1) an indispensable tool for scientists investigating these areas. Anti-amylase antibody (A-1) recognizes both precursor and mature forms of amylase, enhancing utility in studying protein processing and maturation. Additionally, amylase (A-1) monoclonal antibody is available in both non-conjugated and various conjugated forms, including horseradish peroxidase (HRP), phycoerythrin (PE), and fluorescein isothiocyanate (FITC), providing flexibility for diverse experimental applications.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Amylase Antibody (A-1)

sc-514229
200 µg/ml
$316.00

Amylase Antibody (A-1): m-IgG Fc BP-HRP Bundle

sc-538604
200 µg Ab; 10 µg BP
$354.00

Amylase Antibody (A-1): m-IgGκ BP-HRP Bundle

sc-536248
200 µg Ab; 40 µg BP
$354.00

Amylase Antibody (A-1): m-IgG1 BP-HRP Bundle

sc-545791
200 µg Ab; 20 µg BP
$354.00

Amylase (A-1) Neutralizing Peptide

sc-514229 P
100 µg/0.5 ml
$68.00

What application is the blocking peptide sc-514229 P appropriate for?

Asked by: Trav11
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
  • y_2025, m_9, d_26, h_7CST
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Rated 5 out of 5 by from WB: positive results in human salivaWB: positive results in human saliva. -SCBT QC
Date published: 2015-03-15
Rated 5 out of 5 by from Works great for human Amylase transfected lysatesWorks great for human Amylase transfected lysates. -SCBT QC
Date published: 2015-02-06
Rated 4 out of 5 by from Overblown signal in western blot analysisOverblown signal in western blot analysis of Amylase expression in mouse pancreas and rat pancreas tissue extracts. -SCBT QC
Date published: 2013-03-30
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Amylase Antibody (A-1) is rated 4.7 out of 5 by 3.
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