Amylase Antibody (G-10): sc-46657

Amylase Antibody (G-10) is a mouse monoclonal IgG2a kappa light chain antibody engineered to detect amylase proteins across multiple species, including mouse, rat, and human. anti-Amylase antibody (G-10) targets amino acids 212-492 of human Amylase 2B, also known as AMY2B, and effectively recognizes key isoforms such as Amylase 1A (AMY1A) and Amylase 2A (AMY2A). Amylase enzymes play a pivotal role in carbohydrate metabolism by catalyzing the breakdown of starches into simpler sugars, which is essential for proper digestive function and energy production. Understanding the expression and regulation of amylase is crucial for researching digestive disorders, pancreatic health, and certain ovarian tumors, making Amylase (G-10) antibody an invaluable tool in these studies. Amylase (G-10) monoclonal antibody performs reliably in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Available in both non-conjugated form and various conjugated variants, including horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, anti-Amylase antibody (G-10) accommodates a wide range of experimental workflows. In humans, amylase exists in different haplotypes that influence the number of salivary and pancreatic isoforms, while in mice, distinct expression patterns in the salivary glands and liver highlight its physiological significance. By providing robust detection of amylase isoforms, Amylase (G-10) monoclonal antibody supports researchers in unraveling the complexities of metabolic pathways, enzyme functions, and disease mechanisms related to amylase activity.

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Amylase Antibody (G-10) References:

1. 'Pseudoascites' secondary to an amylase-producing serous ovarian cystadenoma. A case study.  |  Brophy, CM., et al. 1989. J Clin Gastroenterol. 11: 703-6. PMID: 2479672
2. The human alpha-amylase multigene family consists of haplotypes with variable numbers of genes.  |  Groot, PC., et al. 1989. Genomics. 5: 29-42. PMID: 2788608
3. Plant α-amylase inhibitors and their effect on the utilization of polysaccharides contained in the diet.  |  Kurhajec, S. and Franc, A. 2019. Ceska Slov Farm. 68: 148-156. PMID: 31822107
4. Characterization of amylases produced by tumors.  |  Takeuchi, T., et al. 1981. Clin Chem. 27: 556-9. PMID: 6162591
5. Tissue specific expression of mouse alpha-amylase genes.  |  Schibler, U., et al. 1982. Adv Exp Med Biol. 158: 381-5. PMID: 6186131
6. Alpha-amylase inhibitors.  |  Taylor, RH. and Barker, HM. 1983. Lancet. 1: 1228-9. PMID: 6190054
7. Amylase from human serous ovarian tumors: purification and characterization.  |  Zakowski, JJ., et al. 1984. Clin Chem. 30: 62-8. PMID: 6197207
8. Amylase in diagnosis of pancreatic disease.  |  Berk, JE. 1978. Ann Intern Med. 88: 838-9. PMID: 666145
9. Regulation and cloning of the gene encoding amylase activity of the ruminal bacterium Streptococcus bovis.  |  Cotta, MA. and Whitehead, TR. 1993. Appl Environ Microbiol. 59: 189-96. PMID: 7679887
10. The remarkable evolutionary history of the human amylase genes.  |  Meisler, MH. and Ting, CN. 1993. Crit Rev Oral Biol Med. 4: 503-9. PMID: 7690604
11. The effect of starvation on the diurnal variation of amylase secretion from rat parotid glands.  |  Ishikawa, Y., et al. 1993. Res Exp Med (Berl). 193: 255-62. PMID: 7694345
12. Amylase activity of Aspergillus strains--producers of organic acids.  |  Tsekova, K., et al. 1993. Acta Microbiol Bulg. 30: 47-50. PMID: 8285132

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Amylase Antibody (G-10) has 89 citations in a variety of scientific publications.

Primary antibodies like Anti-Amylase Antibody (G-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Amylase Antibody (G-10) Specifications

Alternate Product Name: Amylase

Product Clone Name: G-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 212-492

Epitope Target: Amylase 2B

Epitope Species: human

Product Reactivity/Detection: Amylase 1A, 2A and 2B

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 89 citations in various scientific publications

Available Conjugates/Forms for Amylase Antibody (G-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin