AMID/AIFM2/FSP1 Antibody (B-6): sc-377120

AMID Antibody (B-6) is a mouse monoclonal IgG2a kappa light chain antibody that detects AMID protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). AMID (B-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. AMID protein, also known as apoptosis-inducing factor (AIF)-like mitochondrion-associated inducer of death or p53-responsive gene 3 (PRG3), plays a crucial role in apoptosis as a caspase-independent pro-apoptotic flavoprotein with NAD(P)H oxidase activity. AMID protein is primarily localized in the cytosol and associates with the outer mitochondrial membrane, which allows effective mediation of apoptotic signals in response to cellular stress. AMID protein is expressed in most normal tissues and shows upregulated expression by tumor suppressor protein p53, highlighting its importance in cancer biology. Two isoforms of AMID protein exist due to alternative splicing, with isoform 1 being the full-length protein and isoform 2 lacking amino acids 99-138 while having an additional three amino acids inserted after residue 206. AMID protein overexpression has been linked to apoptosis induction, making AMID protein critical in the study of cell death mechanisms and potential therapeutic targets in cancer treatment.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

AMID/AIFM2/FSP1 Antibody (B-6) References:

1. AMID, an apoptosis-inducing factor-homologous mitochondrion-associated protein, induces caspase-independent apoptosis.  |  Wu, M., et al. 2002. J Biol Chem. 277: 25617-23. PMID: 11980907
2. Apoptosis-inducing factor and apoptosis.  |  Lü, CX., et al. 2003. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 35: 881-5. PMID: 14515203
3. A mitochondrial protein, Bit1, mediates apoptosis regulated by integrins and Groucho/TLE corepressors.  |  Jan, Y., et al. 2004. Cell. 116: 751-62. PMID: 15006356
4. AMID is a p53-inducible gene downregulated in tumors.  |  Wu, M., et al. 2004. Oncogene. 23: 6815-9. PMID: 15273740
5. The human apoptosis-inducing protein AMID is an oxidoreductase with a modified flavin cofactor and DNA binding activity.  |  Marshall, KR., et al. 2005. J Biol Chem. 280: 30735-40. PMID: 15958387
6. The p53-inducible apoptotic protein AMID is not required for normal development and tumor suppression.  |  Mei, J., et al. 2006. Oncogene. 25: 849-56. PMID: 16186796
7. Endoplasmic reticulum mediated necrosis-like apoptosis of HeLa cells induced by Ca2+ oscillation.  |  Hu, Q., et al. 2005. J Biochem Mol Biol. 38: 709-16. PMID: 16336787
8. Yeast AMID homologue Ndi1p displays respiration-restricted apoptotic activity and is involved in chronological aging.  |  Li, W., et al. 2006. Mol Biol Cell. 17: 1802-11. PMID: 16436509
9. Bioinformatic and image analyses of the cellular localization of the apoptotic proteins endonuclease G, AIF, and AMID during apoptosis in human cells.  |  Varecha, M., et al. 2007. Apoptosis. 12: 1155-71. PMID: 17347867

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-AMID/AIFM2/FSP1 Antibody (B-6) has 36 citations in a variety of scientific publications.

Primary antibodies like Anti-AMID/AIFM2/FSP1 Antibody (B-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

AMID/AIFM2/FSP1 Antibody (B-6) Specifications

Alternate Product Name: AMID

Product Clone Name: B-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-300

Epitope Target: AMID

Epitope Species: human

Product Reactivity/Detection: AMID

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 36 citations in various scientific publications

Available Conjugates/Forms for AMID/AIFM2/FSP1 Antibody (B-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin