alpha Synuclein Antibody (D-10): sc-515879

α-synuclein Antibody (D-10) is a mouse monoclonal IgG2a kappa light chain antibody designed to detect α-synuclein, also known as SNCA or NACP, in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA) applications. α-synuclein monoclonal antibody (D-10) specifically recognizes an epitope mapping between amino acids 112-140 at the C-terminus of α-synuclein, a region critical for the protein′s structural integrity and interactions. α-synuclein is an intrinsically disordered protein that can adopt multiple conformations, including alpha-helical structures when bound to membranes and beta-sheet-rich fibrils that are associated with pathological aggregates. The C-terminal region plays a significant role in regulating α-synuclein′s propensity to aggregate, which is a key factor in the development of neurodegenerative diseases such as Parkinson′s disease and Alzheimer′s disease. Structural modifications and interactions within this region influence α-synuclein′s normal function in synaptic vesicle trafficking and neurotransmitter release, which are essential for effective neuronal communication and plasticity. Understanding the structural dynamics of α-synuclein is crucial for elucidating the mechanisms underlying aggregation and the resultant cellular toxicity observed in synucleinopathies. anti-α-synuclein antibody (D-10) facilitates detailed structural studies by enabling precise detection of α-synuclein within various experimental contexts, thereby supporting research aimed at uncovering the molecular basis of function and dysfunction. By targeting the C-terminal region, α-synuclein (D-10) antibody assists in investigating how structural changes in α-synuclein contribute to maintaining neuronal health and the progression of neurodegenerative disorders.

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alpha Synuclein Antibody (D-10) References:

1. Methionine oxidation, alpha-synuclein and Parkinson's disease.  |  Glaser, CB., et al. 2005. Biochim Biophys Acta. 1703: 157-69. PMID: 15680224
2. The role of alpha-synuclein in neurodegenerative diseases.  |  Bennett, MC. 2005. Pharmacol Ther. 105: 311-31. PMID: 15737408
3. The behavior of alpha-synuclein in neurons.  |  Fortin, DL., et al. 2010. Mov Disord. 25 Suppl 1: S21-6. PMID: 20187244
4. α-Synuclein fate: proteasome or autophagy?  |  Engelender, S. 2012. Autophagy. 8: 418-20. PMID: 22361583
5. α-Synuclein aggregation, seeding and inhibition by scyllo-inositol.  |  Ibrahim, T. and McLaurin, J. 2016. Biochem Biophys Res Commun. 469: 529-34. PMID: 26697752
6. Extracellular α-synuclein levels are regulated by neuronal activity.  |  Yamada, K. and Iwatsubo, T. 2018. Mol Neurodegener. 13: 9. PMID: 29467003
7. Alpha synuclein in hematopoiesis and immunity.  |  Pei, Y. and Maitta, RW. 2019. Heliyon. 5: e02590. PMID: 31692680
8. Interplay between tau and α-synuclein liquid-liquid phase separation.  |  Siegert, A., et al. 2021. Protein Sci. 30: 1326-1336. PMID: 33452693
9. Chicago sky blue 6B inhibits α-synuclein aggregation and propagation.  |  Min, JO., et al. 2022. Mol Brain. 15: 27. PMID: 35346306
10. Processing α-synuclein interactions.  |  Yates, D. 2022. Nat Rev Neurosci. 23: 456-457. PMID: 35789327

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-alpha Synuclein Antibody (D-10) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-alpha Synuclein Antibody (D-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

alpha Synuclein Antibody (D-10) Specifications

Alternate Product Name: alpha synuclein

Product Clone Name: D-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 112-140

Epitope Target: α-synuclein

Epitope Species: human

Product Reactivity/Detection: α-synuclein

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for alpha Synuclein Antibody (D-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, B: Biotin, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred alpha Synuclein antibody is alpha Synuclein Antibody (211): sc-12767.