alpha-synuclein Antibody (211) recommended for detection of α-synuclein of human origin by WB, IP, IF and IHC(P)

α-synuclein Antibody (211): sc-12767

α-synuclein Antibody (211) is rated 4.7 out of 5 by 21.
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Datasheets

    • Anti-α-synuclein Antibody (211) is a mouse monoclonal IgG1 (kappa light chain) α-synuclein antibody provided at 200 µg/ml
    • raised against amino acid 121-125 of α-synuclein of human origin
    • alpha-synuclein Antibody (211) is recommended for detection of α-synuclein of human origin by WB, IP, IF and IHC(P)
    • Anti-alpha-synuclein Antibody (211) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of α-synuclein (211): sc-12767.
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I noticed, that you've replaced the sc-7011-R antibody against aSyn with this new 211 ab. Does also the new ab recognize mouse aSyn and work in immunocytochemistry? Is it possible to get a small aliquote of the new ab for testing? Thanks!

Asked by: AnnPan
Thank you for your question. We do not currently have any data in mouse samples using the (211) clone, although this clone has been published in mouse, and some customer reviews indicate positive results in mouse samples. The antibody is recommended for IF and IHC(P) on human samples. We also offer a variety of other α-synuclein antibodies. Please contact our Technical Service Department for a recommendation for the best antibodies for your research applications, and for information about our antibody sample program.
Answered by: Technical Support
Date published: 2019-08-02

Why does my WB result always nebulous?My picture can't reveal the monomeric alpha-synuclein , how to manage it?

Asked by: Sophia W
Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2019-07-13

Can't see any monomeric alpha-synuclein in western blot with this antibody (alpha-synuclein 211), how to deal with it?

Asked by: acegik412
Thank you for your question. Please contact our Technical Service Department or your local distributor for assistance in troubleshooting this antibody.
Answered by: Tech Service
Date published: 2019-03-19

I want to know, this antibody will detect total alpha-syn or monomeric alpha-syn or oligomeric alpha-syn?

Asked by: Amrendra
Thank you for the question. This clone was raised against amino acid 121-125 of α-synuclein of human origin. It does detect the target protein under denaturing conditions in a Western blot as well as in applications like IHC(P). We have not yet determined if the level of reactivity with oligomeric alpha-syn would be different compared to the monomeric alpha-syn.
Answered by: Technical Support Europe
Date published: 2017-12-27

Which secondary reagent do your recommend to be used with α-synuclein Antibody (211): sc-12767 for immunohistochemistry on paraffin embedded sections?

Asked by: jenni
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Santa Cruz TS
Date published: 2016-12-14

My samples are of mouse origin. Will α-synuclein Antibody (211): sc-12767 work with my samples by Western blot?

Asked by: jenni
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Santa Cruz TS
Date published: 2019-03-18
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Rated 5 out of 5 by from α-Synuclein Ab in Glia Journal We used the α-Synuclein antibody from Santa Cruz (sc-12767) on a paper titled "Cx3cr1-deficiency exacerbates alpha-synuclein-A53T induced neuroinflammation and neurodegeneration in a mouse model of Parkinson's disease" and its results in IF using mouse tissue were very satisfactory.
Date published: 2019-06-03
Rated 5 out of 5 by from good antibody for western blotting Using mouse brain tissue for western blotting, it worked well using 1:500 dilution.
Date published: 2018-04-03
Rated 5 out of 5 by from good for IHC cytoplasmic staining, positive in control cells, negative in α-synuclein KO cells.
Date published: 2018-02-13
Rated 5 out of 5 by from Very good antibody WB test for mouse brain sample, ~20kD band and dimmer band, clear; IHC test for mouse PFA fixed cryostat section, very clear signal coming from some cell, blood cells.
Date published: 2018-02-09
Rated 1 out of 5 by from Nessun segnale Ho utilizzato un'aliquota in omaggio di questo anticorpo su neuroni umani derivati da iPSCs e non ho riscontrato alcun segnale in corrispondenza di 19 kDa; ho invece osservato un segnale aspecifico tra 50 kDa e 37 kDa.
Date published: 2018-01-09
Rated 5 out of 5 by from Great Use for experiment Item was purchased for research purposes and end user was pleased.
Date published: 2017-11-06
Rated 5 out of 5 by from WB analysis We analysis the expression of target proteins in brain tissue of Parkinson patients by using alpha -synuclein antibody,which shows alpha -synuclein is a good marker for Parkinson detection.Recommend 1:500 for WB analysis.
Date published: 2017-09-16
Rated 5 out of 5 by from effective antibody Alpha -synuclein antibody is very effective in studying the development of mammary gland hyperplasia ,great bands at 19kda (1:800 dilution).
Date published: 2017-09-16
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