alpha / beta-synuclein Antibody (Syn 202) recommended for detection of α-synuclein and β-synuclein of mouse, rat and human origin by WB, IP, IF and IHC(P)

α/β-synuclein Antibody (Syn 202): sc-32281

α/β-synuclein Antibody (Syn 202) is rated 4.3 out of 5 by 3.
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Datasheets

    • Anti-α/β-synuclein Antibody (Syn 202) is a mouse monoclonal IgG2a (kappa light chain) α/β-synuclein antibody provided at 200 µg/ml
    • raised against human recombinant α/β-synuclein
    • Anti-alpha / beta-synuclein Antibody (Syn 202) is recommended for detection of α-synuclein and β-synuclein of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • Anti-alpha / beta-synuclein Antibody (Syn 202) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of α/β-synuclein (Syn 202): sc-32281.
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What is the epitope recognised?

Asked by: chiara9227
Thank you for your question. The antibody was raised against recombinant human a/b synuclein. The recognition epitope is located within the aas 130-140 of a synuclein with protein accession number https://www.ncbi.nlm.nih.gov/protein/P37840 Please contact our Technical Service by phone or email or by Live Chat if you have any further questions.
Answered by: SCBT Heidelberg Support
Date published: 2018-11-27

I am using α/β-synuclein (Syn 202): sc-32281 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Cweed
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from WB: Worked well in human RA WB: Worked well in human RA-differentiated 3D5 cells. -SCBT Publication Review
Date published: 2015-05-12
Rated 5 out of 5 by from Specificity of this antibody was demonstrated Specificity of this antibody was demonstrated by WB by comparing human -synuclein transfected 293T cells with those that have not been transfected to over-express this protein. -SCBT QC
Date published: 2015-03-11
Rated 4 out of 5 by from Blowout band at expected molecular weight with SK Blowout band at expected molecular weight with SK-N-MC whole cell lysate and mouse brain tissue extract. -SCBT QC
Date published: 2014-04-23
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