α-Adaptin 1 Antibody (C-5): sc-398024

α-Adaptin 1 Antibody (C-5) is a mouse monoclonal IgG2b kappa light chain antibody that detects α-Adaptin 1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). α-Adaptin 1 (C-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. α-Adaptin 1 plays a crucial role in the formation of clathrin-coated vesicles, which are essential for receptor-mediated endocytosis, a process that allows cells to internalize nutrients and signaling molecules. α-Adaptin 1 is primarily located at the plasma membrane, where α-Adaptin 1 interacts with clathrin and other adaptor proteins to facilitate the budding of vesicles from the membrane. Proper functioning of α-Adaptin 1 is vital for maintaining cellular homeostasis and regulating the uptake of various ligands, which can impact numerous physiological processes, including cell signaling and nutrient absorption. α-Adaptin 1′s interaction with other components of the clathrin-mediated endocytic pathway underscores α-Adaptin 1′s importance in cellular transport mechanisms. anti-α-Adaptin 1 antibody (C-5) is an invaluable tool for researchers studying endocytosis and related cellular processes, providing insights into the dynamics of vesicle trafficking and the molecular underpinnings of cellular communication.

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α-Adaptin 1 Antibody (C-5) References:

1. Expression of the adaptor protein m-Numb in mouse male germ cells.  |  Corallini, S., et al. 2006. Reproduction. 132: 887-97. PMID: 17127749
2. Conservation and diversity in families of coated vesicle adaptins.  |  Ponnambalam, S., et al. 1990. J Biol Chem. 265: 4814-20. PMID: 1969413
3. Cloning and expression of gamma-adaptin, a component of clathrin-coated vesicles associated with the Golgi apparatus.  |  Robinson, MS. 1990. J Cell Biol. 111: 2319-26. PMID: 2126014
4. The clathrin adaptor complex AP-2 mediates endocytosis of brassinosteroid insensitive1 in Arabidopsis.  |  Di Rubbo, S., et al. 2013. Plant Cell. 25: 2986-97. PMID: 23975899
5. Structural and functional division into two domains of the large (100- to 115-kDa) chains of the clathrin-associated protein complex AP-2.  |  Kirchhausen, T., et al. 1989. Proc Natl Acad Sci U S A. 86: 2612-6. PMID: 2495531
6. Cloning of cDNAs encoding two related 100-kD coated vesicle proteins (alpha-adaptins).  |  Robinson, MS. 1989. J Cell Biol. 108: 833-42. PMID: 2564002
7. Purification and Characterization of Schwann Cells from Adult Human Skin and Nerve.  |  Stratton, JA., et al. 2017. eNeuro. 4: PMID: 28512649
8. MYO1F regulates antifungal immunity by regulating acetylation of microtubules.  |  Sun, W., et al. 2021. Proc Natl Acad Sci U S A. 118: PMID: 34301894
9. Expression and localization of alpha-adaptin isoforms.  |  Ball, CL., et al. 1995. J Cell Sci. 108 (Pt 8): 2865-75. PMID: 7593326
10. Clathrin assembly protein AP180: primary structure, domain organization and identification of a clathrin binding site.  |  Morris, SA., et al. 1993. EMBO J. 12: 667-75. PMID: 8440257
11. The t(10;11)(p13;q14) in the U937 cell line results in the fusion of the AF10 gene and CALM, encoding a new member of the AP-3 clathrin assembly protein family.  |  Dreyling, MH., et al. 1996. Proc Natl Acad Sci U S A. 93: 4804-9. PMID: 8643484
12. Endocytosis and molecular sorting.  |  Mellman, I. 1996. Annu Rev Cell Dev Biol. 12: 575-625. PMID: 8970738

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-α-Adaptin 1 Antibody (C-5) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-α-Adaptin 1 Antibody (C-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

α-Adaptin 1 Antibody (C-5) Specifications

Alternate Product Name: AP2A1

Product Clone Name: C-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 954-977

Epitope Target: α-Adaptin 1

Epitope Species: mouse

Product Reactivity/Detection: α-Adaptin 1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for α-Adaptin 1 Antibody (C-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred α-Adaptin 1/2 antibody is α-Adaptin 1/2 Antibody (C-8): sc-17771.