α-Adaptin 1/2 Antibody (C-8): sc-17771

α-Adaptin 1/2 Antibody (C-8) is a mouse monoclonal IgG2a kappa light chain antibody that detects α-Adaptin 1/2 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). α-Adaptin 1/2 (C-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. α-Adaptin plays a crucial role in the formation of clathrin-coated vesicles, which are essential for receptor-mediated endocytosis, a process that allows cells to internalize nutrients and signaling molecules. α-Adaptin is primarily located at the plasma membrane and is a key component of the AP-2 complex, which is responsible for mediating vesicle transport from the plasma membrane. The interaction of α-Adaptin with clathrin and other adaptor proteins is vital for the proper assembly of clathrin-coated pits, ensuring efficient endocytosis and cellular uptake of essential substances. Additionally, the presence of splice variants of α-Adaptin in different tissues highlights α-Adaptin′s functional diversity and importance in various cellular contexts. By utilizing anti-α-Adaptin 1/2 antibody (C-8), researchers can gain insights into the mechanisms of endocytosis and the role of α-Adaptin in cellular transport processes.

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α-Adaptin 1/2 Antibody (C-8) References:

1. A structural explanation for the binding of multiple ligands by the alpha-adaptin appendage domain.  |  Owen, DJ., et al. 1999. Cell. 97: 805-15. PMID: 10380931
2. Dual interaction of synaptotagmin with mu2- and alpha-adaptin facilitates clathrin-coated pit nucleation.  |  Haucke, V., et al. 2000. EMBO J. 19: 6011-9. PMID: 11080148
3. Tensile stress induces alpha-adaptin C production in mouse calvariae in an organ culture: possible involvement of endocytosis in mechanical stress-stimulated osteoblast differentiation.  |  Shimomura, J., et al. 2003. J Cell Physiol. 195: 488-96. PMID: 12704659
4. Identification of a family of endocytic proteins that define a new alpha-adaptin ear-binding motif.  |  Ritter, B., et al. 2003. EMBO Rep. 4: 1089-95. PMID: 14555962
5. HM1.24 is internalized from lipid rafts by clathrin-mediated endocytosis through interaction with alpha-adaptin.  |  Masuyama, N., et al. 2009. J Biol Chem. 284: 15927-41. PMID: 19359243
6. Dynamin photoinactivation blocks Clathrin and α-adaptin recruitment and induces bulk membrane retrieval.  |  Kasprowicz, J., et al. 2014. J Cell Biol. 204: 1141-56. PMID: 24662566
7. Structural determinants controlling 14-3-3 recruitment to the endocytic adaptor Numb and dissociation of the Numb·α-adaptin complex.  |  Chen, X., et al. 2018. J Biol Chem. 293: 4149-4158. PMID: 29382713
8. An internally eGFP-tagged α-adaptin is a fully functional and improved fiduciary marker for clathrin-coated pit dynamics.  |  Mino, RE., et al. 2020. Traffic. 21: 603-616. PMID: 32657003
9. Expression and localization of alpha-adaptin isoforms.  |  Ball, CL., et al. 1995. J Cell Sci. 108 (Pt 8): 2865-75. PMID: 7593326
10. The ear of alpha-adaptin interacts with the COOH-terminal domain of the Eps 15 protein.  |  Benmerah, A., et al. 1996. J Biol Chem. 271: 12111-6. PMID: 8662627

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-α-Adaptin 1/2 Antibody (C-8) has 24 citations in a variety of scientific publications.

Primary antibodies like Anti-α-Adaptin 1/2 Antibody (C-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

α-Adaptin 1/2 Antibody (C-8) Specifications

Alternate Product Name: AP2A2

Product Clone Name: C-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 678-977

Epitope Target: α-Adaptin 2

Epitope Species: mouse

Product Reactivity/Detection: α-Adaptin 1 and α-Adaptin 2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 24 citations in various scientific publications

Available Conjugates/Forms for α-Adaptin 1/2 Antibody (C-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin