Alpha 4 Antibody (B-5): sc-373719
- Alpha 4 Antibody (B-5) is a mouse monoclonal IgG2b κ Alpha 4 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 259-294 near the C-terminus of Alpha 4 of human origin
- Alpha 4 Antibody (B-5) is recommended for detection of Alpha 4 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Alpha 4 Antibody (B-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Alpha 4 Antibody (B-5) for WB applications. These reagents are now offered in bundles with Alpha 4 Antibody (B-5) (see ordering information below).
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Alpha 4 Antibody (B-5) is a mouse monoclonal IgG2b kappa light chain antibody that detects Alpha 4 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Alpha 4 (B-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Alpha 4 is a cytoplasmic protein that plays a crucial role in regulating signal transduction by associating with the surface IgM receptor. This interaction is vital for modulating the catalytic activity of type 2A-related serine/threonine phosphatases, specifically protein phosphatase 2A (PP2A). PP2A regulation is significant because of its involvement in various cellular processes, including cell growth, division, and apoptosis. Alpha 4 interacts with MID1, a protein linked to X-linked Opitz GBBB syndrome, where mutations can lead to developmental defects such as craniofacial, cardiac, and genital anomalies. Alpha 4 expression is most pronounced in heart, skeletal muscle, and pancreas tissues, and belongs to the IGBP1/TAP42 family, underscoring its importance in cellular signaling pathways and potential implications in developmental disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Alpha 4 Antibody (B-5) References:
- Phosphorylation and microtubule association of the Opitz syndrome protein mid-1 is regulated by protein phosphatase 2A via binding to the regulatory subunit alpha 4. | Liu, J., et al. 2001. Proc Natl Acad Sci U S A. 98: 6650-5. PMID: 11371618
- MID1, mutated in Opitz syndrome, encodes an ubiquitin ligase that targets phosphatase 2A for degradation. | Trockenbacher, A., et al. 2001. Nat Genet. 29: 287-94. PMID: 11685209
- MID1 and MID2 homo- and heterodimerise to tether the rapamycin-sensitive PP2A regulatory subunit, alpha 4, to microtubules: implications for the clinical variability of X-linked Opitz GBBB syndrome and other developmental disorders. | Short, KM., et al. 2002. BMC Cell Biol. 3: 1. PMID: 11806752
- Developmental expression of alpha4 protein phosphatase regulatory subunit in tissues affected by Opitz syndrome. | Everett, AD. and Brautigan, DL. 2002. Dev Dyn. 224: 461-4. PMID: 12203739
- A new X-linked syndrome with agenesis of the corpus callosum, mental retardation, coloboma, micrognathia, and a mutation in the Alpha 4 gene at Xq13. | Graham, JM., et al. 2003. Am J Med Genet A. 123A: 37-44. PMID: 14556245
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Alpha 4 Antibody (B-5) | sc-373719 | 200 µg/ml | $322.00 | |||
Alpha 4 Antibody (B-5): m-IgGκ BP-HRP Bundle | sc-522469 | 200 µg Ab, 40 µg BP | $361.00 | |||
Alpha 4 Antibody (B-5): m-IgG2b BP-HRP Bundle | sc-549036 | 200 µg Ab; 10 µg BP | $361.00 | |||
Alpha 4 Antibody (B-5) AC | sc-373719 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Alpha 4 Antibody (B-5) HRP | sc-373719 HRP | 200 µg/ml | $322.00 | |||
Alpha 4 Antibody (B-5) FITC | sc-373719 FITC | 200 µg/ml | $336.00 | |||
Alpha 4 Antibody (B-5) PE | sc-373719 PE | 200 µg/ml | $349.00 | |||
Alpha 4 Antibody (B-5) Alexa Fluor® 488 | sc-373719 AF488 | 200 µg/ml | $364.00 | |||
Alpha 4 Antibody (B-5) Alexa Fluor® 546 | sc-373719 AF546 | 200 µg/ml | $364.00 | |||
Alpha 4 Antibody (B-5) Alexa Fluor® 594 | sc-373719 AF594 | 200 µg/ml | $364.00 | |||
Alpha 4 Antibody (B-5) Alexa Fluor® 647 | sc-373719 AF647 | 200 µg/ml | $364.00 | |||
Alpha 4 Antibody (B-5) Alexa Fluor® 680 | sc-373719 AF680 | 200 µg/ml | $364.00 | |||
Alpha 4 Antibody (B-5) Alexa Fluor® 790 | sc-373719 AF790 | 200 µg/ml | $364.00 | |||
Alpha 4 (B-5) Neutralizing Peptide | sc-373719 P | 100 µg/0.5 ml | $69.00 |