Alpha 4 Antibody (5F6): sc-81608

Alpha 4 Antibody (5F6) is a mouse monoclonal IgG1 antibody that detects Alpha 4 in mouse, rat, and human samples through applications such as western blotting (WB) and immunoprecipitation (IP). Alpha 4 (5F6) antibody recognizes a crucial cytoplasmic protein that plays a significant role in regulating signal transduction by associating with the surface IgM receptor. This interaction is vital for modulating the catalytic activity of type 2A-related serine/threonine phosphatases, specifically PP2A, which is essential for various cellular processes, including cell growth and differentiation. Notably, anti-Alpha 4 antibody (5F6) targets a protein that interacts with MID1, whose mutation is linked to X-linked Opitz GBBB syndrome, a condition characterized by a range of developmental defects such as craniofacial, cardiac, and genital anomalies. The accumulation of PP2Ac, a subunit of PP2A, occurs due to impaired E3 ubiquitin ligase activity of MID1, which normally facilitates the degradation of PP2Ac by binding to Alpha 4. Alpha 4 monoclonal antibody (5F6) detects a protein predominantly expressed in the heart, skeletal muscle, and pancreas, and belongs to the IGBP1/TAP42 family, underscoring its relevance in both health and disease.

The preferred Alpha 4 antibody is Alpha 4 Antibody (B-5): sc-373719.