Aldehyde dehydrogenase 5-A1/SSADH/ALDH5A1 Antibody (D-3): sc-390754

ALDH5A1 Antibody (D-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects ALDH5A1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). ALDH5A1 (D-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ALDH5A1 protein, also known as succinate semialdehyde dehydrogenase or SSADH, is a crucial enzyme that plays a significant role in the metabolism of gamma-aminobutyric acid (GABA), a key neurotransmitter in the central nervous system. By facilitating the conversion of succinate semialdehyde to succinate, ALDH5A1 is essential for GABA recycling from the synaptic cleft, thereby maintaining proper neurotransmitter levels and ensuring effective synaptic transmission. This function is particularly important for regulating neuronal excitability and preventing neurological disorders. Mutations in the ALDH5A1 gene can lead to succinate semialdehyde dehydrogenase deficiency, a condition characterized by severe ataxia and developmental delays, highlighting ALDH5A1′s critical role in brain function.

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Aldehyde dehydrogenase 5-A1/SSADH/ALDH5A1 Antibody (D-3) References:

1. High-level expression and characterization of the recombinant enzyme, and tissue distribution of human succinic semialdehyde dehydrogenase.  |  Kang, JH., et al. 2005. Protein Expr Purif. 44: 16-22. PMID: 16199352
2. Succinate semialdehyde dehydrogenase deficiency does not down-regulate gamma-hydroxybutyric acid binding sites in the mouse brain.  |  Mehta, AK., et al. 2006. Mol Genet Metab. 88: 86-9. PMID: 16406647
3. Increased guanidino species in murine and human succinate semialdehyde dehydrogenase (SSADH) deficiency.  |  Jansen, EE., et al. 2006. Biochim Biophys Acta. 1762: 494-8. PMID: 16504488
4. SSADH variation in primates: intra- and interspecific data on a gene with a potential role in human cognitive functions.  |  Blasi, P., et al. 2006. J Mol Evol. 63: 54-68. PMID: 16786440
5. Lipid abnormalities in succinate semialdehyde dehydrogenase (Aldh5a1-/-) deficient mouse brain provide additional evidence for myelin alterations.  |  Barcelo-Coblijn, G., et al. 2007. Biochim Biophys Acta. 1772: 556-62. PMID: 17300923
6. Enzymatic and metabolic evidence for a region specific mitochondrial dysfunction in brains of murine succinic semialdehyde dehydrogenase deficiency (Aldh5a1-/- mice).  |  Sauer, SW., et al. 2007. Neurochem Int. 50: 653-9. PMID: 17303287
7. A human derived SSADH coding variant is replacing the ancestral allele shared with primates.  |  Leone, O., et al. 2006. Ann Hum Biol. 33: 593-603. PMID: 17381057
8. Therapeutic concepts in succinate semialdehyde dehydrogenase (SSADH; ALDH5a1) deficiency (gamma-hydroxybutyric aciduria). Hypotheses evolved from 25 years of patient evaluation, studies in Aldh5a1-/- mice and characterization of gamma-hydroxybutyric acid pharmacology.  |  Knerr, I., et al. 2007. J Inherit Metab Dis. 30: 279-94. PMID: 17457693
9. Comparative genomics of aldehyde dehydrogenase 5a1 (succinate semialdehyde dehydrogenase) and accumulation of gamma-hydroxybutyrate associated with its deficiency.  |  Malaspina, P., et al. 2009. Hum Genomics. 3: 106-20. PMID: 19164088
10. Temporal metabolomics in dried bloodspots suggests multipathway disruptions in aldh5a1-/- mice, a model of succinic semialdehyde dehydrogenase deficiency.  |  Brown, M., et al. 2019. Mol Genet Metab. 128: 397-408. PMID: 31699650
11. ALDH5A1 acts as a tumour promoter and has a prognostic impact in papillary thyroid carcinoma.  |  Deng, XY., et al. 2021. Cell Biochem Funct. 39: 317-325. PMID: 32881051
12. ALDH5A1/miR-210 axis plays a key role in reprogramming cellular metabolism and has a significant correlation with glioblastoma patient survival.  |  Mondal, I., et al. 2024. Cancer Cell Int. 24: 259. PMID: 39039535

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Aldehyde dehydrogenase 5-A1/SSADH/ALDH5A1 Antibody (D-3) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-Aldehyde dehydrogenase 5-A1/SSADH/ALDH5A1 Antibody (D-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Aldehyde dehydrogenase 5-A1/SSADH/ALDH5A1 Antibody (D-3) Specifications

Alternate Product Name: ALDH5A1

Product Clone Name: D-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-226

Epitope Target: ALDH5A1

Epitope Species: human

Product Reactivity/Detection: ALDH5A1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for Aldehyde dehydrogenase 5-A1/SSADH/ALDH5A1 Antibody (D-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin