Aiolos Antibody (H-8): sc-515913
- Aiolos Antibody (H-8) is a mouse monoclonal IgG2b κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 85-104 within an internal region of Aiolos of human origin
- Aiolos Antibody (H-8) is recommended for detection of Aiolos isoforms 1-6 of human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Aiolos Antibody (H-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Aiolos Antibody (H-8) for WB and IHC(P) applications. These reagents are now offered in bundles with Aiolos Antibody (H-8) (see ordering information below).
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SEE ALSO...
Aiolos Antibody (H-8) is a mouse monoclonal IgG2b antibody that detects Aiolos of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-Aiolos antibody (H-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Aiolos, also known as zinc finger protein Ikaros 3 or ZNFN1A3, is a crucial 509 amino acid nuclear protein that plays a significant role in lymphocyte differentiation regulation. Mutations in Aiolos can lead to leukemogenesis, highlighting its importance in hematopoiesis and immune system integrity. Predominantly expressed in the spleen, thymus, and peripheral blood leukocytes, Aiolos contains six C2H2-type zinc fingers, a structural motif essential for DNA binding and forming both homodimers and heterodimers with other Ikaros family members, such as Ikaros and Helios. These interactions are critical for transcriptional regulation of genes involved in immune cell development and function. Aiolos (H-8) antibody is an invaluable tool for researchers studying immune regulation complexities and molecular mechanisms underlying hematological malignancies.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Aiolos Antibody (H-8) References:
- Aiolos and Lymphocyte Mimicry in Lung Cancer. | Terada, LS. and Liu, Z. 2014. Mol Cell Oncol. 1: e29912. PMID: 27308319
- Overexpression of Aiolos promotes epithelial-mesenchymal transition and cancer stem cell-like properties in lung cancer cells. | Hung, JJ., et al. 2019. Sci Rep. 9: 2991. PMID: 30816208
- N160 of Aiolos Determines its DNA-Binding Activity. | Wang, H., et al. 2019. Anat Rec (Hoboken). 302: 2014-2019. PMID: 31251838
- Inborn errors of IKAROS and AIOLOS. | Yamashita, M. and Morio, T. 2021. Curr Opin Immunol. 72: 239-248. PMID: 34265590
- Tissue signals imprint Aiolos expression in ILC2s to modulate type 2 immunity. | Qiu, J., et al. 2021. Mucosal Immunol. 14: 1306-1322. PMID: 34349237
- Aiolos represses CD4+ T cell cytotoxic programming via reciprocal regulation of TFH transcription factors and IL-2 sensitivity. | Read, KA., et al. 2023. Nat Commun. 14: 1652. PMID: 36964178
- IKAROS and AIOLOS directly regulate AP-1 transcriptional complexes and are essential for NK cell development. | Goh, W., et al. 2024. Nat Immunol. 25: 240-255. PMID: 38182668
- Kidins220 and Aiolos promote thymic iNKT cell development by reducing TCR signals. | Herr, LA., et al. 2024. Sci Adv. 10: eadj2802. PMID: 38489359
- Targeting Ikaros and Aiolos: reviewing novel protein degraders for the treatment of multiple myeloma, with a focus on iberdomide and mezigdomide. | Liu, Y., et al. 2024. Expert Rev Hematol. 17: 445-465. PMID: 39054911
- Transcriptional function of E2A, Ebf1, Pax5, Ikaros and Aiolos analyzed by in vivo acute protein degradation in early B cell development. | Fedl, AS., et al. 2024. Nat Immunol. 25: 1663-1677. PMID: 39179932
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Aiolos Antibody (H-8) | sc-515913 | 200 µg/ml | $322.00 | |||
Aiolos Antibody (H-8): m-IgG Fc BP-HRP Bundle | sc-531557 | 200 µg Ab; 10 µg BP | $361.00 | |||
Aiolos Antibody (H-8): m-IgGκ BP-HRP Bundle | sc-525281 | 200 µg Ab, 40 µg BP | $361.00 | |||
Aiolos Antibody (H-8): m-IgG2b BP-HRP Bundle | sc-550299 | 200 µg Ab; 10 µg BP | $361.00 | |||
Aiolos Antibody (H-8) AC | sc-515913 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Aiolos Antibody (H-8) HRP | sc-515913 HRP | 200 µg/ml | $322.00 | |||
Aiolos Antibody (H-8) FITC | sc-515913 FITC | 200 µg/ml | $336.00 | |||
Aiolos Antibody (H-8) PE | sc-515913 PE | 200 µg/ml | $349.00 | |||
Aiolos Antibody (H-8) Alexa Fluor® 488 | sc-515913 AF488 | 200 µg/ml | $364.00 | |||
Aiolos Antibody (H-8) Alexa Fluor® 546 | sc-515913 AF546 | 200 µg/ml | $364.00 | |||
Aiolos Antibody (H-8) Alexa Fluor® 594 | sc-515913 AF594 | 200 µg/ml | $364.00 | |||
Aiolos Antibody (H-8) Alexa Fluor® 647 | sc-515913 AF647 | 200 µg/ml | $364.00 | |||
Aiolos Antibody (H-8) Alexa Fluor® 680 | sc-515913 AF680 | 200 µg/ml | $364.00 | |||
Aiolos Antibody (H-8) Alexa Fluor® 790 | sc-515913 AF790 | 200 µg/ml | $364.00 |