AIF Antibody (E-1): sc-13116

AIF Antibody (E-1) is a mouse monoclonal IgG2b kappa light chain antibody raised against amino acids 1-300 of human Apoptosis-Inducing Factor (AIF), also known as AIFM1. AIF (E-1) antibody detects AIF proteins from mouse, rat, and human origins in applications such as western blotting, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin-embedded), flow cytometry (FCM), and ELISA. AIF is a mitochondrial flavoprotein that plays a critical role in caspase-independent apoptosis by translocating from the mitochondria to the nucleus upon apoptotic stimuli. In the nucleus, AIF induces chromatin condensation and large-scale DNA fragmentation, contributing to programmed cell death. Understanding AIF′s function is important because AIF provides insights into the mechanisms of cell death, which has implications in neurodegenerative diseases, cancer, and other pathologies where apoptosis is dysregulated. Additionally, AIF is involved in mitochondrial energy metabolism and maintenance of the mitochondrial respiratory chain. Mutations in the AIFM1 gene, which encodes AIF, have been associated with human diseases such as X-linked recessive mitochondrial encephalopathy. By targeting the N-terminal region of AIF, AIF (E-1) antibody is a valuable tool for researchers investigating the role of AIF in apoptosis and mitochondrial function. AIF monoclonal antibody (E-1) enables reliable detection of AIF in various experimental applications, aiding in the advancement of research in cell death and mitochondrial biology.

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AIF Antibody (E-1) References:

1. Apoptosis-inducing factor (AIF): a novel caspase-independent death effector released from mitochondria.  |  Candé, C., et al. 2002. Biochimie. 84: 215-22. PMID: 12022952
2. Bcl-2-family proteins and the role of mitochondria in apoptosis.  |  Kuwana, T. and Newmeyer, DD. 2003. Curr Opin Cell Biol. 15: 691-9. PMID: 14644193
3. Down-regulation of human RNA/DNA helicase SUV3 induces apoptosis by a caspase- and AIF-dependent pathway.  |  Szczesny, RJ., et al. 2007. Biol Cell. 99: 323-32. PMID: 17352692
4. Caspase-independent death of human osteosarcoma cells by flavonoids is driven by p53-mediated mitochondrial stress and nuclear translocation of AIF and endonuclease G.  |  Kook, SH., et al. 2007. Apoptosis. 12: 1289-98. PMID: 17356895
5. Apoptosis-Inducing Factor (AIF) in Physiology and Disease: The Tale of a Repented Natural Born Killer.  |  Bano, D. and Prehn, JHM. 2018. EBioMedicine. 30: 29-37. PMID: 29605508
6. Mitochondrial control of nuclear apoptosis.  |  Zamzami, N., et al. 1996. J Exp Med. 183: 1533-44. PMID: 8666911
7. Bcl-2 inhibits the mitochondrial release of an apoptogenic protease.  |  Susin, SA., et al. 1996. J Exp Med. 184: 1331-41. PMID: 8879205
8. The release of cytochrome c from mitochondria: a primary site for Bcl-2 regulation of apoptosis.  |  Kluck, RM., et al. 1997. Science. 275: 1132-6. PMID: 9027315
9. The caspase-3 precursor has a cytosolic and mitochondrial distribution: implications for apoptotic signaling.  |  Mancini, M., et al. 1998. J Cell Biol. 140: 1485-95. PMID: 9508780
10. Mitochondria and apoptosis.  |  Green, DR. and Reed, JC. 1998. Science. 281: 1309-12. PMID: 9721092
11. Mitochondrial release of caspase-2 and -9 during the apoptotic process.  |  Susin, SA., et al. 1999. J Exp Med. 189: 381-94. PMID: 9892620
12. Molecular characterization of mitochondrial apoptosis-inducing factor.  |  Susin, SA., et al. 1999. Nature. 397: 441-6. PMID: 9989411

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-AIF Antibody (E-1) has 269 citations in a variety of scientific publications.

Primary antibodies like Anti-AIF Antibody (E-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

AIF Antibody (E-1) Specifications

Alternate Product Name: AIF

Product Clone Name: E-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-300

Epitope Target: AIF

Epitope Species: human

Product Reactivity/Detection: AIF

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 269 citations in various scientific publications

Available Conjugates/Forms for AIF Antibody (E-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin