AHA-1 Antibody (F-7): sc-166610
- AHA-1 Antibody (F-7) is a mouse monoclonal IgG1 κ AHA-1 antibody, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 249-338 mapping at the C-terminus of AHA-1 of human origin
- AHA-1 Antibody (F-7) is recommended for detection of AHA-1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-AHA-1 Antibody (F-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for AHA-1 Antibody (F-7) for WB applications. These reagents are now offered in bundles with AHA-1 Antibody (F-7) (see ordering information below).
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AHA-1 Antibody (F-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects AHA-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). AHA-1 (F-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. AHA-1 plays a pivotal role in regulating heat shock protein 90 (HSP 90) complex, which is essential for protein folding and stabilization under stress conditions. Specifically, AHA-1 enhances HSP 90 ATPase activity, facilitating function in protein maturation and degradation pathways. Such regulation influences "ATP lid" conformational state and promotes N-terminal dimerization, crucial for HSP 90′s activity. AHA-1 predominantly resides in cytosol but can transiently associate with endoplasmic reticulum, impacting protein trafficking from endoplasmic reticulum to Golgi apparatus. Heat shock induces AHA-1 expression in various tissues, including brain, heart, skeletal muscle, and kidney, with lower levels in liver and placenta. AHA-1′s ability to maintain cell viability under non-optimal growth conditions highlights significance in cellular stress responses, making anti-AHA-1 antibody (F-7) valuable for studying protein homeostasis and stress signaling pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
AHA-1 Antibody (F-7) References:
- Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning. | Hu, RM., et al. 2000. Proc Natl Acad Sci U S A. 97: 9543-8. PMID: 10931946
- Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells. | Zhang, QH., et al. 2000. Genome Res. 10: 1546-60. PMID: 11042152
- p38: A novel protein that associates with the vesicular stomatitis virus glycoprotein. | Sevier, CS. and Machamer, CE. 2001. Biochem Biophys Res Commun. 287: 574-82. PMID: 11554768
- Activation of the ATPase activity of hsp90 by the stress-regulated cochaperone aha1. | Panaretou, B., et al. 2002. Mol Cell. 10: 1307-18. PMID: 12504007
- Aha1 binds to the middle domain of Hsp90, contributes to client protein activation, and stimulates the ATPase activity of the molecular chaperone. | Lotz, GP., et al. 2003. J Biol Chem. 278: 17228-35. PMID: 12604615
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AHA-1 Antibody (F-7) | sc-166610 | 200 µg/ml | $322.00 | |||
AHA-1 Antibody (F-7): m-IgG Fc BP-HRP Bundle | sc-529004 | 200 µg Ab; 10 µg BP | $361.00 | |||
AHA-1 Antibody (F-7): m-IgGκ BP-HRP Bundle | sc-521674 | 200 µg Ab, 40 µg BP | $361.00 | |||
AHA-1 Antibody (F-7): m-IgG1 BP-HRP Bundle | sc-543262 | 200 µg Ab; 20 µg BP | $361.00 | |||
AHA-1 Antibody (F-7) AC | sc-166610 AC | 500 µg/ml, 25% agarose | $424.00 | |||
AHA-1 Antibody (F-7) HRP | sc-166610 HRP | 200 µg/ml | $322.00 | |||
AHA-1 Antibody (F-7) FITC | sc-166610 FITC | 200 µg/ml | $336.00 | |||
AHA-1 Antibody (F-7) PE | sc-166610 PE | 200 µg/ml | $349.00 | |||
AHA-1 Antibody (F-7) Alexa Fluor® 488 | sc-166610 AF488 | 200 µg/ml | $364.00 | |||
AHA-1 Antibody (F-7) Alexa Fluor® 546 | sc-166610 AF546 | 200 µg/ml | $364.00 | |||
AHA-1 Antibody (F-7) Alexa Fluor® 594 | sc-166610 AF594 | 200 µg/ml | $364.00 | |||
AHA-1 Antibody (F-7) Alexa Fluor® 647 | sc-166610 AF647 | 200 µg/ml | $364.00 | |||
AHA-1 Antibody (F-7) Alexa Fluor® 680 | sc-166610 AF680 | 200 µg/ml | $364.00 | |||
AHA-1 Antibody (F-7) Alexa Fluor® 790 | sc-166610 AF790 | 200 µg/ml | $364.00 |