AHA-1 Antibody (C-12): sc-166002

AHA-1 Antibody (C-12) is a mouse monoclonal IgG2b light chain antibody that detects AHA-1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). AHA-1 plays a pivotal role in regulating the heat shock protein 90 (HSP 90) complex, which is essential for protein folding, stabilization, and degradation, thereby influencing cellular stress responses. AHA-1 (C-12) antibody′s ability to stimulate the inherent ATPase activity of HSP 90 is crucial for maintaining cell viability under non-optimal growth conditions, particularly when HSP 90 levels are limiting. AHA-1 is primarily located in the cytosol but can transiently interact with the endoplasmic reticulum, impacting the trafficking of proteins from the endoplasmic reticulum to the Golgi apparatus. This interaction is vital for proper cellular function and protein processing. AHA-1 is expressed in various tissues, including the brain, heart, skeletal muscle, and kidney, with lower expression levels in the liver and placenta. Expression is induced by heat shock and treatment with the HSP 90 inhibitor 17-demethoxygeldanamycin, highlighting its importance in stress response mechanisms. Anti-AHA-1 antibody (C-12) is an invaluable tool for researchers studying the dynamics of HSP 90 regulation and its implications in cellular stress responses and related pathologies.

The preferred AHA-1 antibody is AHA-1 Antibody (F-7): sc-166610.