ADO Antibody (E-11): sc-515318
- ADO Antibody (E-11) is a mouse monoclonal IgG2b κ ADO antibody, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 240-258 near the C-terminus of ADO of human origin
- ADO Antibody (E-11) is recommended for detection of ADO of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-ADO Antibody (E-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ADO Antibody (E-11) for WB applications. These reagents are now offered in bundles with ADO Antibody (E-11) (see ordering information below).
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ADO Antibody (E-11) is a mouse monoclonal IgG2b kappa light chain antibody that detects ADO protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ADO Antibody (E-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ADO protein, or 2-aminoethanethiol dioxygenase, plays a crucial role in cysteamine metabolism, converting cysteamine into hypotaurine, which supports cellular antioxidant defense and detoxification processes. This enzymatic activity is vital as hypotaurine serves as a precursor for taurine, a compound essential for various physiological functions, including bile salt formation, osmoregulation, and modulation of calcium signaling. ADO is encoded by a gene located on human chromosome 10q21.3 and consists of 270 amino acids, showing ubiquitous expression with highest levels in brain, heart, and skeletal muscle. ADO function is enhanced by interaction with metal ions, particularly iron, which acts as a cofactor in enzymatic activity. Notably, ADO overexpression in HepG2/C3A cells significantly increases hypotaurine production from cysteamine, while reduced expression leads to decreased levels of this important metabolite, highlighting ADO′s critical role in maintaining cellular homeostasis and metabolic balance.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ADO Antibody (E-11) References:
- Emergence of Talanin protein associated with human uric acid nephrolithiasis in the Hominidae lineage. | Gianfrancesco, F., et al. 2004. Gene. 339: 131-8. PMID: 15363853
- Identification and characterization of the TRIP8 and REEP3 genes on chromosome 10q21.3 as novel candidate genes for autism. | Castermans, D., et al. 2007. Eur J Hum Genet. 15: 422-31. PMID: 17290275
- Genome-wide association study identifies new susceptibility loci for Crohn disease and implicates autophagy in disease pathogenesis. | Rioux, JD., et al. 2007. Nat Genet. 39: 596-604. PMID: 17435756
- Discovery and characterization of a second mammalian thiol dioxygenase, cysteamine dioxygenase. | Dominy, JE., et al. 2007. J Biol Chem. 282: 25189-98. PMID: 17581819
- Mitochondrial dysfunction, oxidative stress, and apoptosis revealed by proteomic and transcriptomic analyses of the striata in two mouse models of Parkinson's disease. | Chin, MH., et al. 2008. J Proteome Res. 7: 666-77. PMID: 18173235
- 3T3-L1 adipocytes and rat adipose tissue have a high capacity for taurine synthesis by the cysteine dioxygenase/cysteinesulfinate decarboxylase and cysteamine dioxygenase pathways. | Ueki, I. and Stipanuk, MH. 2009. J Nutr. 139: 207-14. PMID: 19106324
- Thiol dioxygenases: unique families of cupin proteins. | Stipanuk, MH., et al. 2011. Amino Acids. 41: 91-102. PMID: 20195658
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ADO Antibody (E-11) | sc-515318 | 200 µg/ml | $316.00 | |||
ADO Antibody (E-11): m-IgG Fc BP-HRP Bundle | sc-531337 | 200 µg Ab; 10 µg BP | $354.00 | |||
ADO Antibody (E-11): m-IgGκ BP-HRP Bundle | sc-525002 | 200 µg Ab, 40 µg BP | $354.00 | |||
ADO Antibody (E-11): m-IgG2b BP-HRP Bundle | sc-550279 | 200 µg Ab; 10 µg BP | $354.00 | |||
ADO Antibody (E-11) AC | sc-515318 AC | 500 µg/ml, 25% agarose | $416.00 | |||
ADO Antibody (E-11) HRP | sc-515318 HRP | 200 µg/ml | $316.00 | |||
ADO Antibody (E-11) FITC | sc-515318 FITC | 200 µg/ml | $330.00 | |||
ADO Antibody (E-11) PE | sc-515318 PE | 200 µg/ml | $343.00 | |||
ADO Antibody (E-11) Alexa Fluor® 488 | sc-515318 AF488 | 200 µg/ml | $357.00 | |||
ADO Antibody (E-11) Alexa Fluor® 546 | sc-515318 AF546 | 200 µg/ml | $357.00 | |||
ADO Antibody (E-11) Alexa Fluor® 594 | sc-515318 AF594 | 200 µg/ml | $357.00 | |||
ADO Antibody (E-11) Alexa Fluor® 647 | sc-515318 AF647 | 200 µg/ml | $357.00 | |||
ADO Antibody (E-11) Alexa Fluor® 680 | sc-515318 AF680 | 200 µg/ml | $357.00 | |||
ADO Antibody (E-11) Alexa Fluor® 790 | sc-515318 AF790 | 200 µg/ml | $357.00 | |||
ADO (E-11) Neutralizing Peptide | sc-515318 P | 100 µg/0.5 ml | $68.00 |