ACCβ Antibody (H-7): sc-390344
- ACCβ Antibody (H-7) is a mouse monoclonal IgG2b κ, cited in 4 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 87-106 near the N-terminus of ACCβ of rat origin
- Anti-ACC beta Antibody (H-7) is recommended for detection of ACCβ of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-ACC beta Antibody (H-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ACCβ Antibody (H-7) for WB applications. These reagents are now offered in bundles with ACCβ Antibody (H-7) (see ordering information below).
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ACCβ Antibody (H-7) is a mouse monoclonal IgG2b kappa light chain antibody that detects ACCβ in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ACCβ (H-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Acetyl-CoA carboxylase (ACC) plays a crucial role in lipid metabolism by catalyzing the carboxylation of acetyl-CoA to malonyl-CoA, which is the rate-limiting step in fatty acid synthesis. ACCβ regulation is particularly important as malonyl-CoA produced by ACCβ inhibits carnitine-palmitoyl-CoA transferase I, thereby controlling fatty acid entry into mitochondria for oxidation. This regulatory mechanism maintains energy homeostasis, especially during exercise when fatty acid oxidation demand increases. ACCβ is encoded on human chromosome 12 and produces a mitochondrial protein predominantly expressed in heart and skeletal muscle, where ACCβ plays a significant role in energy metabolism. The catalytic core of ACCβ shares structural similarity with ACCα, while a unique additional peptide at the N terminus highlights the functional diversity and specialization of these isoforms in fatty acid metabolism.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ACCβ Antibody (H-7) References:
- Expression, purification, and characterization of human acetyl-CoA carboxylase 2. | Kim, KW., et al. 2007. Protein Expr Purif. 53: 16-23. PMID: 17223360
- Functional single-nucleotide polymorphism in acetyl-CoA carboxylase ACACB gene promoter. | Lee, AK., et al. 2010. DNA Cell Biol. 29: 703-12. PMID: 20799892
- The effect of ACACB cis-variants on gene expression and metabolic traits. | Ma, L., et al. 2011. PLoS One. 6: e23860. PMID: 21887335
- Biotin augments acetyl CoA carboxylase 2 gene expression in the hypothalamus, leading to the suppression of food intake in mice. | Sone, H., et al. 2016. Biochem Biophys Res Commun. 476: 134-9. PMID: 27181349
- A Novel Acetyl-CoA Carboxylase 2 Selective Inhibitor Improves Whole-Body Insulin Resistance and Hyperglycemia in Diabetic Mice through Target-Dependent Pathways. | Takagi, H., et al. 2020. J Pharmacol Exp Ther. 372: 256-263. PMID: 31900320
- Fine-Tuning Lipid Metabolism by Targeting Mitochondria-Associated Acetyl-CoA-Carboxylase 2 in BRAFV600E Papillary Thyroid Carcinoma. | Valvo, V., et al. 2021. Thyroid. 31: 1335-1358. PMID: 33107403
- Faster lipid β-oxidation rate by acetyl-CoA carboxylase 2 inhibition alleviates high-glucose-induced insulin resistance via SIRT1/PGC-1α in human podocytes. | Wang, Q., et al. 2021. J Biochem Mol Toxicol. 35: e22797. PMID: 33957017
- Acetyl-CoA carboxylase 2 inhibition reduces skeletal muscle bioactive lipid content and attenuates progression of type 2 diabetes in Zucker diabetic fatty rats. | Takagi, H., et al. 2021. Eur J Pharmacol. 910: 174451. PMID: 34454928
- ACACB is a novel metabolism-related biomarker in the prediction of response to cetuximab therapy inmetastatic colorectal cancer. | Hong, HJ., et al. 2022. Acta Biochim Biophys Sin (Shanghai). 54: 1671-1683. PMID: 36111743
- Roles of acetyl-CoA carboxylase beta in muscle cell differentiation and in mitochondrial fatty acid oxidation. | Lee, JK. and Kim, KH. 1999. Biochem Biophys Res Commun. 254: 657-60. PMID: 9920796
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ACCβ Antibody (H-7) | sc-390344 | 200 µg/ml | $316.00 | |||
ACCβ Antibody (H-7): m-IgGκ BP-HRP Bundle | sc-523499 | 200 µg Ab, 40 µg BP | $354.00 | |||
ACCβ Antibody (H-7): m-IgG2b BP-HRP Bundle | sc-549121 | 200 µg Ab; 10 µg BP | $354.00 | |||
ACCβ Antibody (H-7) AC | sc-390344 AC | 500 µg/ml, 25% agarose | $416.00 | |||
ACCβ Antibody (H-7) HRP | sc-390344 HRP | 200 µg/ml | $316.00 | |||
ACCβ Antibody (H-7) FITC | sc-390344 FITC | 200 µg/ml | $330.00 | |||
ACCβ Antibody (H-7) PE | sc-390344 PE | 200 µg/ml | $343.00 | |||
ACCβ Antibody (H-7) Alexa Fluor® 488 | sc-390344 AF488 | 200 µg/ml | $357.00 | |||
ACCβ Antibody (H-7) Alexa Fluor® 546 | sc-390344 AF546 | 200 µg/ml | $357.00 | |||
ACCβ Antibody (H-7) Alexa Fluor® 594 | sc-390344 AF594 | 200 µg/ml | $357.00 | |||
ACCβ Antibody (H-7) Alexa Fluor® 647 | sc-390344 AF647 | 200 µg/ml | $357.00 | |||
ACCβ Antibody (H-7) Alexa Fluor® 680 | sc-390344 AF680 | 200 µg/ml | $357.00 | |||
ACCβ Antibody (H-7) Alexa Fluor® 790 | sc-390344 AF790 | 200 µg/ml | $357.00 | |||
ACCβ (H-7) Neutralizing Peptide | sc-390344 P | 100 µg/0.5 ml | $68.00 |