AASS Antibody (H-5): sc-390511
- AASS Antibody (H-5) is a mouse monoclonal IgG2a κ AASS antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 231-253 of AASS of human origin
- AASS Antibody (H-5) is recommended for detection of AASS of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- Anti-AASS Antibody (H-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for AASS Antibody (H-5) for WB and IHC(P) applications. These reagents are now offered in bundles with AASS Antibody (H-5) (see ordering information below).
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AASS Antibody (H-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects AASS protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). AASS (H-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The alpha-aminoadipic semialdehyde synthase (AASS) protein, also known as lysine ketoglutarate reductase (LKR) or saccharopine dehydrogenase (SDH), is a crucial 926 amino acid enzyme that functions as a homodimer within the mitochondria. AASS plays a vital role in lysine metabolism, catalyzing the initial steps of lysine degradation, which is essential for maintaining amino acid balance and preventing toxic accumulation. AASS enzyme′s activity is particularly important in the liver, where AASS is highly expressed, and AASS gene transcription is upregulated during periods of starvation, highlighting AASS role in metabolic adaptation. Mutations in the AASS gene can lead to familial hyperlysinemias, a group of autosomal recessive disorders characterized by elevated lysine levels, lysinuria, and variable saccharopinuria; however, patients with hyperlysinemia typically do not exhibit significant mental or physical health issues.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
AASS Antibody (H-5) References:
- Identification of the alpha-aminoadipic semialdehyde synthase gene, which is defective in familial hyperlysinemia. | Sacksteder, KA., et al. 2000. Am J Hum Genet. 66: 1736-43. PMID: 10775527
- Identification of the alpha-aminoadipic semialdehyde dehydrogenase-phosphopantetheinyl transferase gene, the human ortholog of the yeast LYS5 gene. | Praphanphoj, V., et al. 2001. Mol Genet Metab. 72: 336-42. PMID: 11286508
- Alpha-aminoadipate delta-semialdehyde synthase mRNA knockdown reduces the lysine requirement of a mouse hepatic cell line. | Cleveland, BM., et al. 2008. J Nutr. 138: 2143-7. PMID: 18936211
- Inhibition of creatine kinase activity by lysine in rat cerebral cortex. | Tonin, AM., et al. 2009. Metab Brain Dis. 24: 349-60. PMID: 19370404
- Familial hyperlysinemias. Purification and characterization of the bifunctional aminoadipic semialdehyde synthase with lysine-ketoglutarate reductase and saccharopine dehydrogenase activities. | Markovitz, PJ., et al. 1984. J Biol Chem. 259: 11643-6. PMID: 6434529
- Multiple enzyme defects in familial hyperlysinemia. | Dancis, J., et al. 1976. Pediatr Res. 10: 686-91. PMID: 934735
- [Familial hyperlysinemia(alpha-aminoadipic semialdehyde synthase defect)]. | Oyanagi, K. and Nagao, M. 1998. Ryoikibetsu Shokogun Shirizu. 188-90. PMID: 9590024
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AASS Antibody (H-5) | sc-390511 | 200 µg/ml | $322.00 | |||
AASS Antibody (H-5): m-IgG Fc BP-HRP Bundle | sc-530176 | 200 µg Ab; 10 µg BP | $361.00 | |||
AASS Antibody (H-5): m-IgGκ BP-HRP Bundle | sc-523564 | 200 µg Ab, 40 µg BP | $361.00 | |||
AASS Antibody (H-5): m-IgG2a BP-HRP Bundle | sc-547451 | 200 µg Ab; 10 µg BP | $361.00 | |||
AASS Antibody (H-5) AC | sc-390511 AC | 500 µg/ml, 25% agarose | $424.00 | |||
AASS Antibody (H-5) HRP | sc-390511 HRP | 200 µg/ml | $322.00 | |||
AASS Antibody (H-5) FITC | sc-390511 FITC | 200 µg/ml | $336.00 | |||
AASS Antibody (H-5) PE | sc-390511 PE | 200 µg/ml | $349.00 | |||
AASS Antibody (H-5) Alexa Fluor® 488 | sc-390511 AF488 | 200 µg/ml | $364.00 | |||
AASS Antibody (H-5) Alexa Fluor® 546 | sc-390511 AF546 | 200 µg/ml | $364.00 | |||
AASS Antibody (H-5) Alexa Fluor® 594 | sc-390511 AF594 | 200 µg/ml | $364.00 | |||
AASS Antibody (H-5) Alexa Fluor® 647 | sc-390511 AF647 | 200 µg/ml | $364.00 | |||
AASS Antibody (H-5) Alexa Fluor® 680 | sc-390511 AF680 | 200 µg/ml | $364.00 | |||
AASS Antibody (H-5) Alexa Fluor® 790 | sc-390511 AF790 | 200 µg/ml | $364.00 | |||
AASS (H-5) Neutralizing Peptide | sc-390511 P | 100 µg/0.5 ml | $69.00 |