A20/TNFAIP3 Antibody (A-12): sc-166692

A20 Antibody (A-12) is a mouse monoclonal IgG2a kappa light chain antibody that detects A20 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). A20 (A-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. A20, also known as TNFAIP3 or TNF alpha induced protein 3, is a critical Cys2/Cys2 zinc finger protein that plays a vital role in regulating inflammatory responses. A20 is primarily located in the cytoplasm and is induced by inflammatory stimuli such as tumor necrosis factor (TNF) and interleukin 1 (IL-1). A20 functions as a negative regulator of nuclear factor κ B (NFκB) signaling, which is essential for controlling immune responses and preventing excessive inflammation. By inhibiting NFκB activation, A20 helps terminate inflammatory signals, maintaining cellular homeostasis and preventing potential tissue damage. A20 structure, particularly seven zinc finger domains, is crucial for function, as only four domains are necessary for in vitro inhibition of TNF-induced NFκB activation. A20 interacts with several key proteins, including TRAF2, TRAF6, and IkB kinase (IKK) γ, further underscoring importance in cellular signaling pathways. Discovery of TXBP151, a novel A20-binding protein, suggests A20 may also play a role in mediating anti-apoptotic activities, highlighting potential as a therapeutic target in various diseases characterized by dysregulated inflammation.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

A20/TNFAIP3 Antibody (A-12) References:

1. The zinc finger protein A20 interacts with a novel anti-apoptotic protein which is cleaved by specific caspases.  |  De Valck, D., et al. 1999. Oncogene. 18: 4182-90. PMID: 10435631
2. A20 and A20-binding proteins as cellular inhibitors of nuclear factor-kappa B-dependent gene expression and apoptosis.  |  Beyaert, R., et al. 2000. Biochem Pharmacol. 60: 1143-51. PMID: 11007952
3. A20 zinc finger protein inhibits TNF-induced apoptosis and stress response early in the signaling cascades and independently of binding to TRAF2 or 14-3-3 proteins.  |  Lademann, U., et al. 2001. Cell Death Differ. 8: 265-72. PMID: 11319609
4. Functional redundancy of the zinc fingers of A20 for inhibition of NF-kappaB activation and protein-protein interactions.  |  Klinkenberg, M., et al. 2001. FEBS Lett. 498: 93-7. PMID: 11389905
5. Identification of a novel A20-binding inhibitor of nuclear factor-kappa B activation termed ABIN-2.  |  Van Huffel, S., et al. 2001. J Biol Chem. 276: 30216-23. PMID: 11390377
6. The deubiquitinase A20 in immunopathology of autoimmune diseases.  |  Majumdar, I. and Paul, J. 2014. Autoimmunity. 47: 307-19. PMID: 24673262
7. Emerging roles for A20 in islet biology and pathology.  |  Zammit, NW. and Grey, ST. 2014. Adv Exp Med Biol. 809: 141-62. PMID: 25302370
8. A20 phosphorylation controls A20 function.  |  Martens, A. and van Loo, G. 2019. Nat Immunol. 20: 1261-1262. PMID: 31534239
9. A20 restricts inflammation via ubiquitin binding.  |  Sun, SC. 2020. Nat Immunol. 21: 362-364. PMID: 32205879
10. A20 Attenuates the Fibrotic Response in the Trabecular Meshwork.  |  Mzyk, P., et al. 2022. Int J Mol Sci. 23: PMID: 35216043
11. A20 and the noncanonical NF-κB pathway are key regulators of neutrophil recruitment during fetal ontogeny.  |  Rohwedder, I., et al. 2023. JCI Insight. 8: PMID: 36633909
12. Genetic Mutations Associated With TNFAIP3 (A20) Haploinsufficiency and Their Impact on Inflammatory Diseases.  |  Bagyinszky, E. and An, SSA. 2024. Int J Mol Sci. 25: PMID: 39125844

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-A20/TNFAIP3 Antibody (A-12) has 74 citations in a variety of scientific publications.

Primary antibodies like Anti-A20/TNFAIP3 Antibody (A-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

A20/TNFAIP3 Antibody (A-12) Specifications

Alternate Product Name: TNFAIP3

Product Clone Name: A-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-100

Epitope Target: A20

Epitope Species: human

Product Reactivity/Detection: A20

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 74 citations in various scientific publications

Available Conjugates/Forms for A20/TNFAIP3 Antibody (A-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent