4E-BP3 Antibody (4-RY9): sc-134232

4E-BP3 Antibody (4-RY9) is a mouse monoclonal IgG2a kappa light chain antibody that detects the 4E-BP3 protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-4E-BP3 antibody (4-RY9) is available as a non-conjugated format. The 4E-BP3 protein, also known as EIF4EBP3 (eukaryotic translation initiation factor 4E binding protein 3), plays a crucial role in the regulation of protein synthesis in eukaryotic cells, which is essential for cell growth and proliferation. This 100 amino acid protein is a member of the eIF4E-binding protein family and is primarily expressed in the heart, kidney, pancreas, and skeletal muscle, with lower expression levels in the thymus and brain. 4E-BP3 functions by interacting with eIF4E, thereby inhibiting its incorporation into the eIF4F complex, which is vital for the initiation of translation. This regulatory mechanism is particularly important in response to various cellular signals, including nutrient availability and growth factors, as it helps control the overall protein synthesis rate in the cell. Additionally, 4E-BP3 is subject to post-translational modifications, including phosphorylation, which further modulates its activity and stability, and is encoded by a gene located on human chromosome 5.

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4E-BP3 Antibody (4-RY9) References:

1. Caspase cleavage of initiation factor 4E-binding protein 1 yields a dominant inhibitor of cap-dependent translation and reveals a novel regulatory motif.  |  Tee, AR. and Proud, CG. 2002. Mol Cell Biol. 22: 1674-83. PMID: 11865047
2. Localisation and regulation of the eIF4E-binding protein 4E-BP3.  |  Kleijn, M., et al. 2002. FEBS Lett. 532: 319-23. PMID: 12482586
3. Ser-64 and Ser-111 in PHAS-I are dispensable for insulin-stimulated dissociation from eIF4E.  |  Ferguson, G., et al. 2003. J Biol Chem. 278: 47459-65. PMID: 14507920
4. Gene fusion and overlapping reading frames in the mammalian genes for 4E-BP3 and MASK.  |  Poulin, F., et al. 2003. J Biol Chem. 278: 52290-7. PMID: 14557257
5. Characterization of mammalian eIF4E-family members.  |  Joshi, B., et al. 2004. Eur J Biochem. 271: 2189-203. PMID: 15153109
6. Structural basis for mRNA Cap-Binding regulation of eukaryotic initiation factor 4E by 4E-binding protein, studied by spectroscopic, X-ray crystal structural, and molecular dynamics simulation methods.  |  Tomoo, K., et al. 2005. Biochim Biophys Acta. 1753: 191-208. PMID: 16271312
7. Binding preference of eIF4E for 4E-binding protein isoform and function of eIF4E N-terminal flexible region for interaction, studied by SPR analysis.  |  Abiko, F., et al. 2007. Biochem Biophys Res Commun. 355: 667-72. PMID: 17316564
8. 4E-BP3, a new member of the eukaryotic initiation factor 4E-binding protein family.  |  Poulin, F., et al. 1998. J Biol Chem. 273: 14002-7. PMID: 9593750

The preferred p-4E-BP1/2/3 antibody is p-4E-BP1/2/3 Antibody (A-10): sc-271947.