4E-BP1 Antibody (D-10): sc-514073
- 4E-BP1 Antibody (D-10) is a mouse monoclonal IgG2a κ, cited in 3 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 2-25 at the N-terminus of 4E-BP1 of human origin
- recommended for detection of 4E-BP1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- See 4E-BP1 (P-1): sc-9977 for 4E-BP1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for 4E-BP1 Antibody (D-10) for WB and IHC(P) applications. These reagents are now offered in bundles with 4E-BP1 Antibody (D-10) (see ordering information below).
4E-BP1 Antibody (D-10) is a mouse monoclonal IgG2a antibody that detects 4E-BP1 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). 4E-BP1, also known as PHAS-I, plays a crucial role in the regulation of protein synthesis by inhibiting the function of eIF-4E, a key component of the eIF-4F complex that initiates translation. When phosphorylated by kinases such as S6 kinase p70, MAP kinases, or protein kinase C, 4E-BP1 dissociates from eIF-4E, thereby promoting the assembly of the translation initiation complex and enhancing protein synthesis. This regulatory mechanism is vital for processes such as cell growth, proliferation, and survival, making anti-4E-BP1 antibody (D-10) a significant tool for studying cancer biology and other diseases where protein synthesis is dysregulated.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
4E-BP1 Antibody (D-10) References:
- 4E-BP1 is a target of Smad4 essential for TGFbeta-mediated inhibition of cell proliferation. | Azar, R., et al. 2009. EMBO J. 28: 3514-22. PMID: 19834456
- 4E-BP1 participates in maintaining spindle integrity and genomic stability via interacting with PLK1. | Shang, ZF., et al. 2012. Cell Cycle. 11: 3463-71. PMID: 22918237
- 4E-BP1 regulates the sensitivity of human glioma cells to chemotherapy through PI3K/Akt/mTOR-independent pathway. | Zhu, HL., et al. 2014. Neuropathology. 34: 227-35. PMID: 24354477
- New insights into 4E-BP1-regulated translation in cancer progression and metastasis. | Wang, J., et al. 2014. Cancer Cell Microenviron. 1: PMID: 26005705
- The Translational Repressor 4E-BP1 Contributes to Diabetes-Induced Visual Dysfunction. | Miller, WP., et al. 2016. Invest Ophthalmol Vis Sci. 57: 1327-37. PMID: 26998719
- Increased 4E-BP1 Expression Protects against Diet-Induced Obesity and Insulin Resistance in Male Mice. | Tsai, SY., et al. 2016. Cell Rep. 16: 1903-14. PMID: 27498874
- Phosphorylated 4E-BP1 is associated with tumor progression and adverse prognosis in colorectal cancer. | Chen, Y., et al. 2017. Neoplasma. 64: 787-794. PMID: 28592131
- Expression of 4E-BP1 and phospho-4E-BP1 correlates with the prognosis of patients with clear cell renal carcinoma. | Li, F., et al. 2018. Cancer Manag Res. 10: 1553-1563. PMID: 29942157
- 4E-BP1 phosphorylation is mediated by the FRAP-p70s6k pathway and is independent of mitogen-activated protein kinase. | von Manteuffel, SR., et al. 1996. Proc Natl Acad Sci U S A. 93: 4076-80. PMID: 8633019
- 4E-BP1, a repressor of mRNA translation, is phosphorylated and inactivated by the Akt(PKB) signaling pathway. | Gingras, AC., et al. 1998. Genes Dev. 12: 502-13. PMID: 9472019
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
4E-BP1 Antibody (D-10) | sc-514073 | 200 µg/ml | $322.00 | |||
4E-BP1 Antibody (D-10): m-IgG Fc BP-HRP Bundle | sc-538581 | 200 µg Ab; 10 µg BP | $361.00 | |||
4E-BP1 Antibody (D-10): m-IgGκ BP-HRP Bundle | sc-536219 | 200 µg Ab; 40 µg BP | $361.00 | |||
4E-BP1 (D-10) Neutralizing Peptide | sc-514073 P | 100 µg/0.5 ml | $69.00 |