26S Proteasome p58 Antibody (U6/272): sc-65744
- 26S Proteasome p58 Antibody (U6/272) is a mouse monoclonal IgG2a κ 26S Proteasome p58 antibody provided at 200 µg/ml
- raised against 26S Proteasome purified from embryos of Drosophila melanogaster origin
- 26S Proteasome p58 Antibody (U6/272) is recommended for detection of p58 subunit of the 19S regulatory lid complex of the 26S Proteasome of d origin by WB and IP
- Anti-26S Proteasome p58 Antibody (U6/272) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for 26S Proteasome p58 Antibody (U6/272). This work is in progress.
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SEE ALSO...
26S Proteasome p58 Antibody (U6/272) is a mouse monoclonal IgG2a kappa light chain antibody that detects the 26S Proteasome p58 protein of Drosophila melanogaster origin by western blotting (WB) and immunoprecipitation (IP). Anti-26S Proteasome p58 antibody (U6/272) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The 26S proteasome is a crucial multi-subunit complex responsible for the degradation of ubiquitinated proteins, a process vital for maintaining cellular homeostasis and regulating various biological functions such as the cell cycle, apoptosis, and responses to oxidative stress. 26S proteasome p58, also referred to as Rpn3 in yeast and S3 in humans, is one of the essential non-ATPase lid subunits of the 19S regulatory complex, playing a significant role in the assembly and stability of the entire proteasome structure. 26S proteasome p58 is integral to the recognition of ubiquitinated substrates, facilitating the removal of ubiquitin chains and the subsequent translocation of these substrates into the 20S core for degradation. Given its pivotal function in protein turnover and quality control, 26S proteasome p58 is critical for cellular health and function, making 26S proteasome p58 an important target for research in various fields, including cancer biology and neurodegenerative diseases.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
26S Proteasome p58 Antibody (U6/272) References:
- The regulatory complex of Drosophila melanogaster 26S proteasomes. Subunit composition and localization of a deubiquitylating enzyme. | Hölzl, H., et al. 2000. J Cell Biol. 150: 119-30. PMID: 10893261
- Assembly of the Drosophila 26 S proteasome is accompanied by extensive subunit rearrangements. | Kurucz, E., et al. 2002. Biochem J. 365: 527-36. PMID: 11945175
- Genome-wide expression analysis in Drosophila reveals genes controlling circadian behavior. | Ceriani, MF., et al. 2002. J Neurosci. 22: 9305-19. PMID: 12417656
- Proteasomal ATPase-associated factor 1 negatively regulates proteasome activity by interacting with proteasomal ATPases. | Park, Y., et al. 2005. Mol Cell Biol. 25: 3842-53. PMID: 15831487
- Zn2+-induced reversible dissociation of subunit Rpn10/p54 of the Drosophila 26 S proteasome. | Kiss, P., et al. 2005. Biochem J. 391: 301-10. PMID: 15946124
- The proteolytic function of the Arabidopsis 26S proteasome is required for specifying leaf adaxial identity. | Huang, W., et al. 2006. Plant Cell. 18: 2479-92. PMID: 17028202
- Aging perturbs 26S proteasome assembly in Drosophila melanogaster. | Vernace, VA., et al. 2007. FASEB J. 21: 2672-82. PMID: 17413001
- Rpn13p and Rpn14p are involved in the recognition of ubiquitinated Gcn4p by the 26S proteasome. | Seong, KM., et al. 2007. FEBS Lett. 581: 2567-73. PMID: 17499717
- Automated cryoelectron microscopy of 'single particles' applied to the 26S proteasome. | Nickell, S., et al. 2007. FEBS Lett. 581: 2751-6. PMID: 17531228
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
26S Proteasome p58 Antibody (U6/272) | sc-65744 | 200 µg/ml | $322.00 | |||
26S Proteasome p58 Antibody (U6/272) AC | sc-65744 AC | 500 µg/ml, 25% agarose | $424.00 | |||
26S Proteasome p58 Antibody (U6/272) HRP | sc-65744 HRP | 200 µg/ml | $322.00 | |||
26S Proteasome p58 Antibody (U6/272) FITC | sc-65744 FITC | 200 µg/ml | $336.00 | |||
26S Proteasome p58 Antibody (U6/272) PE | sc-65744 PE | 200 µg/ml | $349.00 | |||
26S Proteasome p58 Antibody (U6/272) Alexa Fluor® 488 | sc-65744 AF488 | 200 µg/ml | $364.00 | |||
26S Proteasome p58 Antibody (U6/272) Alexa Fluor® 546 | sc-65744 AF546 | 200 µg/ml | $364.00 | |||
26S Proteasome p58 Antibody (U6/272) Alexa Fluor® 594 | sc-65744 AF594 | 200 µg/ml | $364.00 | |||
26S Proteasome p58 Antibody (U6/272) Alexa Fluor® 647 | sc-65744 AF647 | 200 µg/ml | $364.00 | |||
26S Proteasome p58 Antibody (U6/272) Alexa Fluor® 680 | sc-65744 AF680 | 200 µg/ml | $364.00 | |||
26S Proteasome p58 Antibody (U6/272) Alexa Fluor® 790 | sc-65744 AF790 | 200 µg/ml | $364.00 |