17β-HSD Antibody (D-8): sc-373902
- 17β-HSD Antibody (D-8) is a mouse monoclonal IgG1 κ 17β-HSD antibody, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 171-328 mapping at the C-terminus of 17β-HSD of human origin
- 17 beta-HSD Antibody (D-8) is recommended for detection of 17β-HSD of human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-17 beta-HSD Antibody (D-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for 17β-HSD Antibody (D-8) for WB and IHC(P) applications. These reagents are now offered in bundles with 17β-HSD Antibody (D-8) (see ordering information below).
17β-HSD Antibody (D-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects 17β-HSD protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHC), and enzyme-linked immunosorbent assay (ELISA). 17β-HSD (D-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. 17β-hydroxysteroid dehydrogenase type 1 (17β-HSD) enzyme plays a crucial role in steroid metabolism, catalyzing the conversion of estrone to estradiol and androstenedione to testosterone, which are vital for reproductive health and various physiological processes. 17β-HSD is predominantly expressed in ovarian granulosa cells and breast tissue, but can also be found in testis, placenta, uterus, prostate, and adipose tissue. 17β-HSD′s homodimeric structure allows preferential utilization of NADP(H) for oxidation and reduction reactions, essential for enzymatic activity. Human 17β-HSD gene is located on chromosome 17q12-q21, and regulation is significant in breast cancer, as increased 17β-HSD expression has been associated with poorer outcomes in patients, particularly those treated with tamoxifen. Specific inhibition of 17β-HSD may represent a promising therapeutic strategy in managing breast cancer, highlighting 17β-HSD′s importance in both normal physiology and disease states.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
17β-HSD Antibody (D-8) References:
- Structure and function of 17beta-hydroxysteroid dehydrogenase type 1 and type 2. | Vihko, P., et al. 2001. Mol Cell Endocrinol. 171: 71-6. PMID: 11165013
- Amplification of HSD17B1 and ERBB2 in primary breast cancer. | Gunnarsson, C., et al. 2003. Oncogene. 22: 34-40. PMID: 12527905
- Subunit identity of the dimeric 17 beta-hydroxysteroid dehydrogenase from human placenta. | Lin, SX., et al. 1992. J Biol Chem. 267: 16182-7. PMID: 1322895
- The gene for 17 beta-hydroxysteroid dehydrogenase maps to human chromosome 17, bands q12-q21, and shows an RFLP with ScaI. | Winqvist, R., et al. 1990. Hum Genet. 85: 473-6. PMID: 1977681
- Structure of two in tandem human 17 beta-hydroxysteroid dehydrogenase genes. | Luu-The, V., et al. 1990. Mol Endocrinol. 4: 268-75. PMID: 2330005
- Differential estrogen substrate specificities for transiently expressed human placental 17 beta-hydroxysteroid dehydrogenase and an endogenous enzyme expressed in cultured COS-m6 cells. | Poutanen, M., et al. 1993. Endocrinology. 133: 2639-44. PMID: 8243287
- Characteristics of human types 1, 2 and 3 17 beta-hydroxysteroid dehydrogenase activities: oxidation/reduction and inhibition. | Luu-The, V., et al. 1995. J Steroid Biochem Mol Biol. 55: 581-7. PMID: 8547185
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
17β-HSD Antibody (D-8) | sc-373902 | 200 µg/ml | $322.00 | |||
17β-HSD Antibody (D-8): m-IgG Fc BP-HRP Bundle | sc-529602 | 200 µg Ab; 10 µg BP | $361.00 | |||
17β-HSD Antibody (D-8): m-IgGκ BP-HRP Bundle | sc-522548 | 200 µg Ab, 40 µg BP | $361.00 | |||
17β-HSD Antibody (D-8): m-IgG1 BP-HRP Bundle | sc-543600 | 200 µg Ab; 20 µg BP | $361.00 | |||
17β-HSD Antibody (D-8) AC | sc-373902 AC | 500 µg/ml, 25% agarose | $424.00 | |||
17β-HSD Antibody (D-8) HRP | sc-373902 HRP | 200 µg/ml | $322.00 | |||
17β-HSD Antibody (D-8) FITC | sc-373902 FITC | 200 µg/ml | $336.00 | |||
17β-HSD Antibody (D-8) PE | sc-373902 PE | 200 µg/ml | $349.00 | |||
17β-HSD Antibody (D-8) Alexa Fluor® 488 | sc-373902 AF488 | 200 µg/ml | $364.00 | |||
17β-HSD Antibody (D-8) Alexa Fluor® 546 | sc-373902 AF546 | 200 µg/ml | $364.00 | |||
17β-HSD Antibody (D-8) Alexa Fluor® 594 | sc-373902 AF594 | 200 µg/ml | $364.00 | |||
17β-HSD Antibody (D-8) Alexa Fluor® 647 | sc-373902 AF647 | 200 µg/ml | $364.00 | |||
17β-HSD Antibody (D-8) Alexa Fluor® 680 | sc-373902 AF680 | 200 µg/ml | $364.00 | |||
17β-HSD Antibody (D-8) Alexa Fluor® 790 | sc-373902 AF790 | 200 µg/ml | $364.00 |