17 beta-HSD Antibody (D-8)recommended for detection of 17β-HSD of human origin by WB, IP, IF, IHC(P) and ELISA

17β-HSD Antibody (D-8): sc-373902

17β-HSD Antibody (D-8) is rated 3.7 out of 5 by 3.
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Datasheets

    • 17β-HSD Antibody (D-8) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 171-328 mapping at the C-terminus of 17β-HSD of human origin
    • 17 beta-HSD Antibody (D-8) is recommended for detection of 17β-HSD of human origin by WB, IP, IF, IHC(P) and ELISA
    • 17 beta-HSD Antibody (D-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of 17β-HSD (D-8): sc-373902.
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Can 17β-HSD (D-8): sc-373902 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: TinTin
Thank you for your question. Yes, 17β-HSD (D-8): sc-373902 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-28
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Rated 1 out of 5 by from no luck with porcine samples We tried this antibody (1:500) with porcine testis samples and observed no bands. However, Santa Cruz did send a free alternative.
Date published: 2016-12-01
Rated 5 out of 5 by from Produced great immunoperoxidase cytoplasmic Produced great immunoperoxidase cytoplasmic and nuclear staining in formalin fixed, paraffin-embedded human placenta tissue. -SCBT QC
Date published: 2015-05-17
Rated 5 out of 5 by from Strong band in WB in BT Strong band in WB in BT-20 whole cell lysate. -SCBT QC
Date published: 2015-03-24
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